投菌强化序批式反应器(SBR)脱氮除磷效果及微生物种属分析  

Enhanced nitrogen and phosphorus removal with addition of microbes in sequencing batch reactor and analysis of microbial specie

作　　者：陈晶[1] 周新程 陈萍[1] 邓文[1] 张文艺[1] 

机构地区：[1]常州大学环境与安全工程学院,常州213164

出　　处：《环境化学》2016年第10期2183-2190,共8页Environmental Chemistry

基　　金：国家"水体污染控制与治理"科技重大专项(2012zx07301-001);国家自然科学基金(41571471);常州市和武进区科技支撑项目(CE20155061;WS201521)资助~~

摘　　要：为揭示投加具有反硝化聚磷能力的恶臭假单胞菌(B8)强化序批式反应器(SBR)除污特性和微生物种属,将B8菌液和干粉菌剂分别引入SBR,构成液态型DNPAOs-SBR污水处理系统A和干粉型DNPAOs-SBR污水处理系统B,以不接菌的SBR污水处理系统C为对照,分别考察了在厌氧-缺氧条件下运行的各反应器除污效果.结果表明,各SBR(A、B、C)对COD去除率均达到90%以上;第49—77天时A系统、B系统和C系统NO-3平均去除率分别为61.62%、68.58%和26.72%;第62—77天时A系统、B系统和C系统TP平均去除率分别为53.66%、55.45%和46.61%;投菌强化系统在缺氧段对TP降解过程符合一阶指数衰减动力学模式,在第71天时,A号吸磷动力学系数KP为1.2584,B号吸磷动力学系数KP为2.0379;对SBR内活性污泥菌种16S r DNA测序及Gen Bank BLAST,A系统和B系统中占优势菌种依次是溶血不动杆菌、恶臭假单胞菌和粪产碱菌,而未投菌C系统占优势菌类依次是溶血不动杆菌、粪产碱菌和产碱假单胞菌,表明投加的B8菌在液态型DNPAOs-SBR污水处理系统和干粉型DNPAOs-SBR污水处理系统内得到生长富集,从而成为优势菌群.This paper aimed to investigate the performance and microbial community of denitrifying polyphosphate accumulating bacteria（ Pseudomonas putida B8） in the sequencing batch reactor（ SBR） system. B8 bacterial liquid and B8 bacteria powder were introduced into SBR respectively to construct new type DNPAOs-SBR sewage disposal systems（ liquid type A and dry powder type B）,with a traditional SBR（ type C） as contrast. The performance of these three reactors under anaerobic-anoxic conditions was investigated. The results showed that the chemical oxygen demand（ COD） removal of all SBR reactors was above 90%,while the mean removal rates of nitrate in A,B and C reactors during 49—77 days were 61. 62%,68. 58% and 26. 72%,respectively. In addition,the mean removal rates of total phosphorus（ TP） in A,B and C reactors during 62—77days were 53. 66%,55. 45% and 46. 61%,respectively. The kinetics of TP removal under anoxic conditions in A and B reactors followed the first-order exponential decay model,with the kinetic constants 1. 2584 and 2. 0379 at 71 day,respectively. The polyphosphate accumulating microbial species in SBR were also analyzed,and the dominant bacteria were Actinetobacter haemolyticus,Pseudomonas putida and Alcaligenes faecalis in the system A and B,while the dominant bacteria were Actinetobacter haemolyticus,Alcaligenes faecalis and Pseudomonas alcaligenes in the control group. The denitrifying polyphosphate accumulating microorganism B8 put in the DNPAOs-SBR systems（ A and B reactors） grew well.

关 键 词：反硝化聚磷菌 序批式反应器 DNA提取 菌种鉴定 系统发育 

分 类 号：X703[环境科学与工程—环境工程]