B群链球菌显色培养基在孕产妇B群链球菌筛查中的性能评价  

Evaluation of the performance of chromogenic agar in the screening group B streptococcus from pregnant women

作　　者：张树琛[1] 王杉[1] 朱凤霞[1] 时琰丽[1] 李东[1] 鲁炳怀[1] 

机构地区：[1]民航总医院检验科,北京100123

出　　处：《首都医科大学学报》2016年第5期680-683,共4页Journal of Capital Medical University

基　　金：北京市科委首都临床特色应用研究(Z141107002514036)~~

摘　　要：目的评价B群链球菌(group B streptococcus,GBS)显色培养基在孕产妇中筛查GBS的敏感性、特异性和选择性能。方法收集孕产妇肛门/阴道拭子标本共312例,已知菌株标本9例,分别直接接种或增菌后接种于血平皿和GBS显色培养基,记录鉴定结果。以筛查出的GBS总阳性数为参考,分别比较直接接种和增菌接种时,显色培养基筛查GBS的敏感性和特异性。结果显色培养基对GBS的检出率、敏感性和特异性优于常规血平皿。标本增菌后接种法孵育24 h得到的阳性率为7.05%(22/312)高于直接接种法(6.41%,20/312),对GBS筛查的敏感性高于直接接种法(95.65%vs 86.96%),特异性一致(99.65%)。结论显色培养基对GBS有良好的检出率,判断方法直观简便,对非目标菌群有良好的抑制作用。Objective To evaluate the sensitivity,specificity and selective capability of chromogenic agar in the screening group B streptococcus（ GBS） in the pregnant women. Methods A total of 312 rectum / vaginal swabs,collected from pregnant women,as well as9 known GBS strains,were inoculated on the blood agar plates（ BAP） and chromogenic medium chrom ID Strepto B agar（ STRB） directly and after the selective enrichment,respectively. The screening results of the above-mentioned methods were compared. Results The STRB showed the excellent performance for GBS detection and outperformed BAP. The selective enrichment is an essential step to screen the GBS isolates due to its higher sensitivity（ 95. 65% vs 86. 96%） while both methods have the similar specificity. Conclusion STRB demonstrated good detection rate of GBS for its direct identification and effective inhibition of non-targeted bacteria.

关 键 词：B群链球菌 显色培养基 孕产妇 

分 类 号：R446.5[医药卫生—诊断学]