响应面法优化中华苦荬菜总三萜提取工艺  

Optimation of Extraction of Total Triterpenoids from Ixeris Chinensis(Thunb.)Nakai by Response Surface Methodology

作　　者：程艳刚 荆然[2] 谭金燕 李慧峰[2] 孔祥鹏[2] 裴妙荣[1,2] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]山西中医学院,山西晋中030619

出　　处：《辽宁中医药大学学报》2016年第10期54-56,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：山西中医学院计划项目(2011PY-3)

摘　　要：目的:优选中华苦荬菜总三萜乙醇回流提取工艺。方法:以总三萜提取率为指标,在单因素试验基础上,通过响应面法考察乙醇浓度、液料比、时间对总三萜提取率的影响。结果:最佳提取工艺条件为加10.2倍量80%乙醇85℃回流提取3次,每次2.6 h,总三萜提取率为1.69%,与理论预测值1.72%偏差较小。结论:通过响应面分析法优选中华苦荬菜总三萜提取工艺可行性较好,为其进一步的开发与利用提供实验依据。Objective：To optimize the extraction of total triterpenoids from Ixeris chinensis（Thunb.）Nakai by response surface methodology. Methods：Based on single factor testes,the extraction technology was optimized by response surface methodology with the concentration of ethanol,material-liquid ratio and time as factors and the yield of total triterpenoids as index. Results：The optimized technology were as follows：80% ethanol as solvent,ratio of material to liquid 10.2∶1,time 2.6 hours,temperature 85 ℃,3 times of extraction. The yield of total triterpenoids was 1.69% under optimal condition,the result of trial was agreed with model predictions. Conclusion：The extraction technology is reasonable and feasible,which provide the references and theoretical foundations for further development of Ixeris chinensis（Thunb.）Nakai.

关 键 词：中华苦荬菜 三萜 超声 响应面法 糖尿病 

分 类 号：R284[医药卫生—中药学]