一株黄杆菌低温噬菌体的生物学特征研究  

Biological characteristics of a cold-active bacteriophage against Flavobacteria

作　　者：王宝强[1] 季秀玲[2] 魏云林[2] 王旺田[1] 董志扬[3] 

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]昆明理工大学生命科学与技术学院,云南昆明650500 [3]中国科学院微生物研究所,北京100101

出　　处：《中国微生态学杂志》2016年第10期1117-1122,共6页Chinese Journal of Microecology

基　　金：国家自然科学基金(31360129)

摘　　要：目的对分离自明永冰川地区的1株黄杆菌噬菌体（SWP-8）生物学特性进行研究。方法利用透射电子显微镜（TEM）技术及氯化铯密度梯度离心法对SWP-8进行形态观察、基因组酶切片段长度多态性分析及噬菌体生理特性研究。结果噬菌体SWP-8为肌尾型,在电镜下呈头尾复合型结构,头部呈正多面体立体对称,直径约150nm;尾管长约150nm,直径约20nm,具有收缩性的鞘,头部与尾管结合处具有颈环结构。在4℃时具有感染性,4～25℃均能产生边缘清晰、透明的噬菌斑。pH值耐受范围较广,最适pH值为7.0。最佳感染复数为10.000,潜伏期60min,裂解期90min,裂解效率较高。结论 SWP-8属肌尾噬菌体科（Myoviridae）,是裂解性噬菌体,对氯仿和蛋白酶K较为敏感。基因组为dsDNA,大小约为75kb。Objective To explore the biological characteristics of a cold-active bacteriophage SWP-8against Flavobacteriaisolated from the Mingyong glacier area.Methods The morphology,restriction fragment length polymorphism（RFLP）of genomic DNA and physiological properties of the bacteriophage SWP-8were analyzed by using transmission electron microscopy（TEM）and CsCl density gradient centrifugation techniques.Results The bacteriophage SWP-8had an isometric head（about 150 nm in diameter）and a tail（about 150 nm in length and 20 nm in width）,and a contractile sheath.The head and tail tube junction by a neck ring structure was detected.Physiological analysis showed that SWP-8had infection activity at 4 ℃;clear and transparent plaques were formed on double-layer plates between 4and 25 ℃.The optimal pH of infection was 7.0,and it had a wide range of pH tolerance.The optimum multiplicity of infection（MOI）was 10.000;the latency and lytic period were 60 min and 90 min,respectively,and it had high cracking efficiency.Conclusion SWP-8is a lytic Myoviridae bacteriophage.It is insensitive to chloroform and proteinase K.The genome of SWP-8is consisted of double-stranded DNA of approximately 75 kb.

关 键 词：黄杆菌属 低温噬菌体 生理特性 

分 类 号：Q939.124[生物学—微生物学]