HPLC法测定民族药材青阳参中青阳参苷乙的含量  

Determination of Otophylloside B in Cynanchi Otophylli Radix by HPLC

作　　者：昝珂[1] 张睿[2] 郑健[1] 过立农[1] 马双成[1] 

机构地区：[1]中国食品药品检定研究院,北京100050 [2]首都医科大学中医药学院,北京100069

出　　处：《中国药事》2016年第10期1004-1008,共5页Chinese Pharmaceutical Affairs

基　　金：国家食品药品监督管理总局药化注册司专项:"12种特色民族药材检验方法的示范性研究"

摘　　要：目的：建立反相高效液相法测定民族药材青阳参中青阳参苷乙的含量。方法：采用YMC-Pack ODS-A C18色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈-水（53∶47）,检测波长223 nm,流速为1.0 m L·min-1,柱温为30℃。结果：青阳参苷乙在20.16～1008μg·m L-1范围内线性关系良好,该方法的精密度、稳定性、重复性、加样回收率试验RSD均小于2%,平均加样回收率为97.14%。结论：该方法准确、简便、灵敏度高,可作为青阳参药材的含量测定方法。Objective： To establish a RP-HPLC method to determine the content of otophylloside B in Cynanchi Otophylli Radix. Methods： The separation was performed on a YMC-Pack ODS-A C18 column（4.6 mm×250 mm,5 μm）with acetonitrile-water（53∶47） as mobile phase, the flow rate was 1.0 m L·min-1. The detection UV wavelength was at 223 nm, and the column temperature was 30 ℃. Results： The calibration curve of otophylloside B was linear in the range of 20.16-1008 μg·m L-1, the RSD of precision, solution stability, repeatability, recovery were less than 2%, and the average recovery was 97.14%. Conclusion： The method is simple, rapid and accurate, and can be used for the quality control of Cynanchi Otophylli Radix.

关 键 词：青阳参 HPLC 青阳参苷乙 含量测定 

分 类 号：R927.2[医药卫生—药学]