肝窦内皮细胞诱导T细胞免疫耐受  

T cell immune tolerance induced by LSEC

作　　者：景亚青 刘义[1] 韩菲[2] 袁晶华 李克秋[1] 李光[1] 

机构地区：[1]天津医科大学基础医学院遗传学系,天津300070 [2]天津医科大学第二医院检验科,天津300211

出　　处：《安徽医科大学学报》2016年第11期1569-1572,1578,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家高技术研究发展计划(863计划)(编号:2012AA021003)

摘　　要：目的探讨肝窦内皮细胞(LSEC)对T细胞的耐受作用。方法 CD3和CD28抗体活化的人T细胞和人LSEC混合培养,检测48 h时单独培养和混合培养T细胞的凋亡情况和细胞因子白细胞介素2(IL-2)、白细胞介素4(IL-4)和白细胞介素10(IL-10)的表达量及分泌量。结果和LSEC混合培养的T细胞凋亡率显著高于单独培养的T细胞,混合培养的T细胞中IL-2的表达量没有发生明显变化,IL-4的表达量显著升高,IL-2/IL-4的值降低,IL-10的表达量增加。IL-2、IL-4和IL-10细胞因子分泌情况和表达量一致。结论LSEC可通过降低T细胞数目和改变细胞因子分泌两种途径诱导T细胞产生耐受。Objective To explore the role of LSEC on T cell tolerance. Methods T cell apoptosis, cytokine（ IL- 2, IL-4 and IL-10） expression and secretion of human T cells activated by CD3/CD28 alone and mixed-cultured with human LSEC were detected. Results The ratio of cell apoptosis was higher in mixed-cultured T cells than T cells alone. The gene expressions of IL-4 and IL-10 were higher in mixed-cultured T cells than T ceils alone, while gene expression of IL-2 had no significant difference in mixed-cultured T cells than T cells alone. The cytokine secretion of IL-2, IL-4 and IL-10 was consistent with gene expression. Conclusion LSEC could induce T cell tolerance by decreasing T cell number and altering cytokine secretion.

关 键 词：LSEC T细胞耐受 混合培养 凋亡检测 细胞因子 

分 类 号：R392[医药卫生—免疫学]