Tim3对poly(I∶C)介导的小鼠Kupffer细胞分泌细胞因子的影响  

Tim 3 affecting mice liver Kupffer cells secretion by poly( I ∶ C) activation

作　　者：牛坚[1] 王月[1] 王人颢[1] 刘斌[1] 

机构地区：[1]徐州医学院附属医院普外科,徐州221002

出　　处：《安徽医科大学学报》2016年第11期1584-1589,共6页Acta Universitatis Medicinalis Anhui

基　　金：天晴肝病研究基金(编号:CFHPC20132020);江苏省333高层次人才培养(编号:Ⅲ-2290);徐州市推动科技创新专项资金项目(编号:KC14SX011)

摘　　要：目的探讨T细胞免疫球蛋白及黏蛋白家族-3(Tim3)对poly(I∶C)活化的小鼠肝Kupffer细胞调节作用并探讨其相关机制。方法将真核表达质粒pc DNA3.1-Tim 3转染小鼠肝Kupffer细胞,以Realtime PCR和Western blot法验证Tim 3在小鼠肝Kupffer细胞的表达。通过ELISA法检测质粒pc DNA3.1-Tim 3,并使用Tim 3阻断型抗体和核转录因子kappa B(NF-κB)抑制性配体对poly(I∶C)活化的小鼠肝Kupffer细胞因子[肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)]产生影响,Western blot法检测NF-κB p65、IκBα蛋白表达。结果 Tim 3抑制小鼠肝Kupffer细胞分泌炎性因子TNF-α、IL-6、IL-1β,Western blot结果显示其降低小鼠肝Kupffer细胞NF-κB p65蛋白和提高IκBα蛋白表达。结论 Tim 3通过NF-κB通路参与了poly(I:C)诱导小鼠肝Kupffer细胞活化的调节。Objective To investigate the adjustment role of T cell immunoglobulin and mucin family-3 （Tim 3 ） in Kupffer cells activation and the related mechanism. Methods Transfected the pcDNA3.1-Tim 3 plasmid into Kupffer cells. Tim 3 expression in Kupffer cell was examined by Realtime PCR and Western blot. Plasmid pcDNA3.1-Tim 3, Tim 3 blocking antibodies and NF-κB inhibitory ligands on mice liver Kupffer cell activation factor （TNF-α, IL-1β, IL-6） were monitored by ELISA test. NF-κB and IκBα proteins were examined by Western blot. Results Tim 3 could inhibit Kupffer cells expression of TNF-α, IL-6, IL-1β. The results showed that it reduced the expression of NF-κB p65 protein and increased IκBα involved in the regulation of Kupffer cells activation protein in the mouse liver Kupffer cells. Conclusion Tim 3 is through NF-kappa B and IκBα protein

关 键 词：Tim3 KUPFFER细胞 小鼠 

分 类 号：R392[医药卫生—免疫学]