同型半胱氨酸对实验性结肠炎大鼠血栓调节蛋白和内皮细胞蛋白C受体表达的影响  

Effects of homocysteine on the expression of TM and EPCR in rats colitis

作　　者：陈义[1] 丁少桢 梅俏[1] 刘晓昌[1] 胡静[1] 韩玮[1] 许建明[1] 

机构地区：[1]安徽医科大学第一附属医院消化内科,合肥230022

出　　处：《安徽医科大学学报》2016年第11期1621-1624,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81470809);安徽省自然科学基金(编号:1308085MH146)

摘　　要：目的探讨同型半胱氨酸(Hcy)对实验性结肠炎大鼠结肠黏膜中可溶性血栓调节蛋白(s TM)、蛋白C(PC)、游离蛋白S(f PS)水平及血栓调节蛋白(TM)、内皮细胞蛋白C受体(EPCR)mRNA表达的影响。方法 SD大鼠分为正常组:组1[生理盐水(NS)皮下注射+NS灌肠]、组2(Hcy皮下注射+NS灌肠);TNBS模型组:组1(NS皮下注射+TNBS灌肠)、组2(Hcy皮下注射+TNBS灌肠)。实验结束时采用ELISA法检测大鼠结肠黏膜中s TM、f PS、PC水平,采用RTq PCR法检测大鼠结肠黏膜中TM、EPCR mRNA的表达水平。结果与TNBS模型组1相比,TNBS模型组2大鼠血浆及结肠Hcy水平均显著增高,大鼠结肠黏膜中s TM、PC和f PS水平降低(P<0.05),大鼠结肠黏膜中TM、EPCR mRNA表达水平降低(P<0.05)。结论 Hcy可以加重大鼠结肠炎症损伤,其机制可能是影响PC的抗炎与抗凝功能,引起肠道微循环血栓前状态。Objective To investigate the effects of homocysteine （Hcy） on level of soluble thrombomodulin （ sTM）, protein C （ PC ）, free protein S （fPS） and mRNA expressions of thrombomodulin （TM） and endothelial protein C receptor （EPCR） in rats with experimental colitis. Methods SD rats were divided into 4 groups ： normal group, normal ＋ Hcy group, TNBS/ethanol group, TNBS/ethanol ＋ Hey group. Experimental colitis model with hyperhomocystinemia was established in rats with intracolonic administration of TNBS and subcutaneous injection of Hey. The mRNA expression of TM and EPCR was detected by RT-qPCR method. The levels of sTM, PC and fPS in colon mucosa tissues of rats were measured by ELISA method. Results Compared with the normal group, the levels of Hey in plasma and colon mucosa were increased remarkably in TNBS-induced colitis rats with Hcy injection simultaneously. Furthermore, the levels of sTM, PC, and fPS in colon mueosa were decreased significantly. RT-qPCR method showed significant decrease in mRNA expressions of TM and EPCR in colon mueosa tissues in TNBS/ethanol ＋ Hcy group. Conclusion Hcy could promote pathological damage in TNBS-induced colitis rats, which may be related to its effects on the anti-inflammatory and anticoagulation of PC system, resulting in prothrombosis condition in colonic mucosal microcirculation.

关 键 词：同型半胱氨酸 可溶性血栓调节蛋白 蛋白C 游离蛋白S 内皮细胞蛋白C受体 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学] R322[医药卫生—基础医学]