茶皂素对瘤胃微生物发酵及原虫数量的影响  

Tea Saponin Affecting Rumen Microbial Fermentation and Number of Protozoa

作　　者：常肖肖 刘续航 赵士萍[1] 蒋琪辉 张议夫 方洛云[1] 蒋林树[1] 

机构地区：[1]奶牛营养学北京市重点实验室,北京农学院动物科技学院,北京102206

出　　处：《中国农学通报》2016年第29期17-21,共5页Chinese Agricultural Science Bulletin

基　　金：2012年度北京市人力社保局“新世纪百千万人才工程资助项目”;2011-2014年北京市自然科学基金(6122006);2014年度北京市教育委员会“北京市属高等学校高层次人才引进与培养计划项目”(CIT&TCD20130324);北京市农业局“北京市现代农业产业技术体系奶牛创新团队”

摘　　要：为研究茶皂素对西门塔尔牛瘤胃微生物发酵及原虫数量的影响,采用4×4拉丁方试验设计,其中茶皂素浓度分为对照组、低剂量组、中剂量组和高剂量组,每头每天分别为0、6、12、18 g,共4期,每期20天,并在试验第20天进行采样。测定瘤胃液p H、挥发性脂肪酸浓度、氨态氮浓度及原虫计数。结果表明:茶皂素对瘤胃挥发性脂肪酸中乙酸和丙酸的比例影响差异显著(P<0.05),第Ⅳ周期由对照组(3.79±0.13)减小到(3.30±0.17)。高、中、低剂量组原虫数量都显著(P<0.05)低于对照组。综上所述,茶皂素对瘤胃中原虫有明显的抑制作用,并影响瘤胃发酵。To study the effect of tea saponin on rumen microbial fermentation and the number of protozoa inSimmental cattle, a 4 × 4 latin square experiment was designed. Concentration of tea saponin in the controlgroup, low dose group, middle dose group and high dose group was 0, 6, 12 and 18 g per cattle in a day,respectively. The experiment lasted for 4 periods and each period lasted for 20 days. p H value, contents ofvolatile fatty acid and ammonia nitrogen, and number of protozoa were measured. Results showed that the ratioof acetic acid and propanoic acid in the control group and high dose group was（3.79±0.13） and（3.30±0.17） inthe fourth period respectively, presenting a significant difference（P〈0.05）. Number of protozoa inexperimental groups were significantly lower than that in the control group（P〈0.05）. In summary, tea saponinhad an obvious inhibitory effect on rumen protozoa, and could significantly affect rumen fermentation.

关 键 词：茶皂素 西门塔尔牛 瘤胃微生物发酵 原虫数量 

分 类 号：S823.94[农业科学—畜牧学]