在水分胁迫下根际促生菌A2对小麦萌芽期和苗期的促生效应  

Growth-Promoting Effects of Rhizosphere Growth Promoting Bacteria A2 on Germination and Seedling Stage of Wheat Under Water Stress

作　　者：刘炳花 萨日娜 葛蓓孛[1] 刘艳[1] 施李鸣[1] 阿拉坦夫 Thinn Thinn Newt 张克诚[1] 

机构地区：[1]中国农业科学院植物保护研究所,北京100193 [2]锡林郭勒盟林木种苗工作站,内蒙古锡林浩特02600 [3]沙河市第一中学,河北沙河054100

出　　处：《中国农学通报》2016年第29期64-69,共6页Chinese Agricultural Science Bulletin

基　　金：国家公益性行业(农业)科研专项"保护地果蔬灰霉病绿色防控技术研究与示范"(201303025);国家自然科学基金"武夷菌素生物合成调控基因的功能研究"(31371985);国家自然科学基因青年基金"wys R3基因调控武夷菌素生物合成的机理研究"(31401796)

摘　　要：为了明确根际促生细菌A2对植物生长的促生效应,通过室内发芽试验,以PEG6000 4个浓度处理条件,对A2菌株3种发酵产物浸种处理的小麦,采用"胚芽鞘长度法"测定种子发芽势、发芽率、胚芽鞘抗旱指数,同时测定其幼苗叶片脯氨酸含量和可溶性糖含量的变化。结果表明PEG6000浓度为4%时不同浸种处理的小麦胚芽鞘长度、发芽率、叶片脯氨酸含量、可溶性糖含量等各项指标均无显著差异,PEG6000浓度为12%时,A2菌株发酵液浸种的小麦发芽率比无菌水对照组高14.93%,其幼苗叶片脯氨酸含量和可溶性糖含量分别比对照高66.55%,66.92%。说明随着PEG6000浓度的增加不同浸种处理间的各项指标差异显著或极显著,A2菌株促进植物生长的同时诱导植物产生抗性物质从而适应不利环境。In order to define the growth-promoting effect of rhizosphere growth promoting bacteria A2 on thegrowth of plant, by indoor germination test, wheat was treated by four different concentrations of PEG6000, andthen soaked in three different fermentation products of A2. The germination potential of seed, rate ofgermination, and index of coleoptile drought resistance were determined by＂Method of Coleoptile Length＂respectively. Meanwhile, the content of proline and soluble sugar were measured in leaf. The results indicatedthat there was no significant difference of coleoptile length, germination rate, proline content and soluble sugarcontent under the concentration of 4% PEG6000. When PEG6000 concentration reached 12%, the germinationrate of wheat was 14.93% higher than that of the control. In addition, the content of proline and soluble sugarwere 66.55% and 66.92% higher than those of the control respectively. In conclusion, with the increasingconcentration of PEG6000, there was significant difference or very significant difference among indexes withseed soaking treatments. So, the A2 promotes the growth of plant and induces plant producing some resistancesubstances to adapt to the unfavorable environment.

关 键 词：小麦 根际促生菌 水分胁迫 促生作用 

分 类 号：S476[农业科学—农业昆虫与害虫防治]