雪莲注射液对大鼠急性实验性胰腺炎的治疗作用  

Therapeutic effects of Saussurea involucrate injection in severe acute pancreatitis in rats

作　　者：王小红[1] 吴孟杰[1] 马文元[2] 魏荣龙 缪青[1] 庄勋[1] 薛祥龙[1] 

机构地区：[1]丹阳市人民医院消化科,江苏省212300 [2]丹阳市人民医院病理科,江苏省212300

出　　处：《中华消化杂志》2016年第10期670-675,共6页Chinese Journal of Digestion

摘　　要：目的 研究雪莲注射液对大鼠重度急性胰腺炎（SAP）的治疗作用.方法 80只健康远交系SD大鼠分为SAP 3 h、48 h组、雪莲处理3h、48 h组、乌司他丁对照3h、48 h组、假手术3h、48 h组,每组10只.造模后SAP组常规饲养,其他3组分别予雪莲注射液（1.04 mL/kg）腹腔注射、乌司他丁10 000 U/L尾静脉注射、0.9％ NaCl溶液股静脉穿刺注射,均为每12 h注射1次.分别在术后第3小时、48小时采集大鼠血液、胰腺组织标本.观察各组死亡率、血清淀粉酶水平和胰腺组织病理学变化.应用酶联免疫吸附法（ELISA）检测血清TNF-α、IL-6和IL-10含量,化学比色法测定胰腺组织丙二醛含量,RT-PCR法检测胰腺组织TNF-α mRNA、IL-6 mRNA和IL-10 mRNA含量,以免疫组织化学法测定胰腺组织NF-κB p65活性.采用单因素方差分析进行多组间比较,组间多重比较采用最小显著差异（LSD）法.率的比较采用Fisher确切概率法.结果 雪莲处理48 h组、SAP 48 h组和乌司他丁48 h组死亡率比较差异均无统计学意义（P均＞0.05）.雪莲处理48 h组胰腺组织的病理组织学评分（8.13±0.64）、血清淀粉酶水平[（2 597.0±214.0）U/L]、TNF-α[（254.4±11.6） ng/L]、IL-6水平[（441.4±14.6） ng/L]、胰腺组织丙二醛含量[（311.0±10.6） mmol/L]、TNF-a mRNA表达水平（2.04±0.08）、IL-6mRNA表达水平（1.77±0.04）、NF-κB p65活性[（25.90±2.90）％]均比SAP组48 h[分别为11.40±0.89、（4 780.0±101.0） U/L、（396.0±7.4） ng/L、（664.4±7.6） ng/L、（418.0±10.6） mmol/L、2.94±0.03、2.63±0.08、（51.60±5.27）％]显著降低（F=235.07、441.63、489.40、2 465.00、196.65、477.89、562.79、131.70,P均＜0.01）,而血清IL-10水平[（133.5±6.9） ng/L比（95.1±5.2） ng/L]和胰腺组织IL-10 mRNA表达水平（1.38±0.06比0.85±0.03）则显著升高（F=560.18、570.04,P均＜0.01）;但与乌司他丁48 h组相比[7.56±0.88、（2 607.0±239.0） U/L、（252.2±9.2） ng/L、�Objective To investigate the therapeutic effects of Saussurea involucrate injection in severe acute pancreatitis （SAP） in rats.Methods A total of 80 healthy Sprague-Dawley rats were divided into eight groups：SAP group （three hours、48 hours）,Saussurea involucrate treated group （three hours、48 hours）,ulinastatin control group （three hours、48 hours） and sham operation group （three hours、48 hours）,10 rats in each group.After modeling,the rats of SAP group were regularly feeded and the rats of other three group were treated with Saussurea involucrate injection （1.04 mL/kg） intraperitoneal injection,ulinastatin 10 000 U/L tail vein injection,and saline femoral vein injection,respectively and injected every 12 hours.At three hours and 48 hours after treated,blood and pancreatic tissue samples were obtained.The mortality rate,serum amylase level and pathological changes of the pancreas of each group were observed.Serum tumor necrosis factor alpha （TNF-α）,interleukin （IL）-6 and IL-10 levels were detected by enzyme linked immunosorbent assay （ELISA）.The content of malondialdehyde （MDA） in pancreatic tissues was determined by chemical colorimetry.The level of TNF-α mRNA,IL 6 mRNA and IL-10 mRNA in pancreatic tissues were measured with reverse trascription-polymerase chain reaction （RT-PCR）.The activity of nuclear factor kappa B p65 （NF-κB p65） in the pancreatic tissue was tested by immunohistochemistry.Single factor analysis of variance was used to compare multiple groups,and the least significant difference （LSD） method was used in the multiple comparisons between groups.Fisher＇s exact probability method was performed for rates comparison.Results At 48 hours,there was no statistically significant difference in mortality rate among Saussurea involucrate treated group,SAP group and ulinastatin groups （all P〉0.05）.At 48 hours,the histopathology score （8.13 ± 0.64）,levels of serum amylase （（2 597.0±214.0） U/L）,TNF-α （（254.4±11.6） ng/

关 键 词：雪莲注射液 乌司他丁 重度急性胰腺炎 

分 类 号：R259[医药卫生—中西医结合]