肝移植后患者外周血Tfh细胞亚群的变化特征及意义  

Characteristic changes and their significance of peripheral follicular helper T cell subsets in liver transplant patients

作　　者：张可[1] 施明[1] 孙艳玲[2] 王立峰[1] 王洪波[2] 刘鸿凌[2] 刘振文[2] 金磊[1] 王福生[1] 

机构地区：[1]北京大学解放军302医院教学医院感染性疾病诊疗与研究中心,北京100039 [2]北京大学解放军302医院教学医院肝移植研究中心,北京100039

出　　处：《解放军医学杂志》2016年第10期833-836,共4页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金(81571567)~~

摘　　要：目的探讨肝移植术前和术后患者外周血滤泡辅助性T细胞(Tfh)亚群的表达变化及其与预后的相关性。方法对11例肝移植术后肝功能稳定的患者进行随访研究,采用流式细胞术检测移植前和移植后4周外周血中Tfh细胞亚群频率的变化。结果与移植术前比较,移植术后4周患者外周血Tfh1(CD4^+CXCR5^+CXCR3^+CCR6–)细胞频率较移植前有下降趋势,但差异无统计学意义(P>0.05),Tfh2(CD4^+CXCR5^+CXCR3–CCR6–)细胞频率较移植前明显升高(P<0.05),Tfh17(CD4^+CXCR5^+CXCR3–CCR6^+)细胞频率较移植前有上升趋势,但差异无统计学意义(P>0.05)。结论 Tfh2和Tfh17细胞可能与肝移植后患者肝功能的恢复有关。Objective To investigate the expression of follicular helper T（Tfh） cell subsets in peripheral blood of patients with liver transplantation（LT） and relevance to the prognosis. Methods Eleven liver transplant patients with stable liver function were enrolled in this study. The frequencies of Tfh subsets were examined by flow cytometry. The frequencies and the level of alanine aminotransferase（ALT）, aspartate transaminase（AST） and total bilirubin（TBIL） were monitored dynamically within one month after LT. Results The frequency of CD4^＋CXCR5^＋CXCR3^-CCR6^- Tfh2 subset in peripheral blood increased significantly after LT as compared with those before transplantation（P〈0.05）. In addition, the frequency of CD4^＋CXCR5^＋CXCR3^–CCR6^＋ Tfh17 subset in peripheral blood showed a trend of increase. The frequency of CD4^＋CXCR5^＋CXCR3^＋CCR6^- Tfh1 subset, however, showed a downtrend, but no statistical difference was found. Conclusion The subsets of Tfh2 and Tfh17 may be involved in the regulation of alloimmune response and play a role in maintaining liver function stability in liver transplant patients.

关 键 词：肝移植 滤泡辅助性T细胞 移植免疫学 

分 类 号：R657.3[医药卫生—外科学]