深港两地工程土壤真菌多样性分析  

Analysis of Fungal Diversity in Engineering Soils from Shenzhen and Hongkong

作　　者：龙海[1,2] 章桂明[1] 汪莹[1,2] 程颖慧[1] 李芳荣[1] 王颖[1] 高瑞芳[1] 李一农[1] 

机构地区：[1]深圳出入境检验检疫局,广东深圳518001 [2]深圳市检验检疫科学研究院深圳市外来有害生物检测技术研发重点实验室,广东深圳518045

出　　处：《安徽农业科学》2016年第27期97-99,116,共4页Journal of Anhui Agricultural Sciences

基　　金：国家质检总局科技计划项目(2015IK266)

摘　　要：[目的]对深圳和香港工程土壤的真菌种类进行检测。[方法]以深圳和香港两地不同深度(0.5、10.0、20.0和30.0 m)的工程土壤为研究对象,结合形态学和ITS序列扩增并测序的方法,分析土壤真菌多样性。[结果]共分离得到934个菌株,分别属于79个属132种。深圳土壤有14个属19种;香港土壤有73个属122种。66个属113种只在香港有分布;7个属10种只在深圳有分布。深港两地均有分布的有7个属9种。[结论]深港两地工程土壤真菌种类有一定差异性;香港工程土壤真菌种类较多,其中还包括多种植物病原真菌,土壤入境前必须进行检疫处理。[Objective] The aim was to detect the fungal diversity in engineering soils from Shenzhen and Hongkong.[Method]The engineering soils of different depths （0.5, 10.0, 20.0 and 30.0 ） in Hongkong and Shenzhen were studied.The fungal diversity in soil was analyzed by the methods of morphological and ITS region amplification and sequencing.[Result]A total of 934 strains were isolated, belonging to 79 genera and 132 species, respectively.There are 14 genera and 19 species in the soil sample of Shenzhen.There are 73 genera and 122 species in that of Hongkong.66 genera, 113 species are distributed only in Hongkong;7 genera, 10 species are distributed only in Shenzhen.7 genera, 9 species are distributed in Shenzhen and Hongong.[Conclusion]The differences of fungal species in engineering soils between Shenzhen and Hongkong are significant.There are many kinds of fungi in the soil of Hongkong project, including many plant pathogenic fungi.Before entering, the soil must be treated with quarantine.

关 键 词：工程土壤 真菌多样性 形态学 分子生物学 

分 类 号：S154.3[农业科学—土壤学]