共振散射光谱法对视黄醇结合蛋白的测定分析  

Detection of Retinol-binding Protein in Serum by Resonance Scattering Spectral Method

作　　者：邹明静[1] 柳彦梅[2] 王娜[2] 

机构地区：[1]菏泽医学专科学校,山东菏泽274030 [2]商丘医学高等专科学校,河南商丘476000

出　　处：《当代化工》2016年第10期2334-2336,共3页Contemporary Chemical Industry

基　　金：商丘市科技攻关项目;项目号:141055;山东省高等学校科技计划项目;项目号:20141012

摘　　要：在pH5.29 Na2HPO4-KH2PO4缓冲溶液中,羊抗人视黄醇结合蛋白与视黄醇结合蛋白（RBP）发生特异性反应生成免疫复合物微粒,导致体系在340 nm处的共振散射峰显著增强。RBP在0.6-360 ng/m L范围内与ΔI340nm存在良好的线性关系,回归方程为ΔI340nm=1.0063CRBP＋9.541,相关系数R为0.996 9,检出限0.1 ng/m L,用于定量分析肾病患者血清中的视黄醇结合蛋白含量,并与免疫透射比浊法比较,结果满意。In Na2HPO4-KH2PO4 buffer solution with p H of 5.29,Retinol Binding protein（RBP） antisera was combined with RBP specifically to produce immunocomplex particles, that resulted in resonance scattering effect at 340 nm.The intensities ΔIRS at 340 nm are proportional to the RBP concentrations from 0.6 ng·m L^-1 to 360 ng·m L^-1. Its regression equation was ΔI340nm=1.0063CRBP＋9.541,the correlation coefficient was 0.9969,and the detection limit was 0.1 ng·m L^-1. The method was successfully applied in quantitative determination for RBP inhuman serum with kidney disease, and had the satisfactory results compared with turbidimetric immunoassay.

关 键 词：视黄醇结合蛋白 共振散射光谱 免疫复合物 

分 类 号：O657[理学—分析化学]