淋巴细胞亚群及免疫球蛋白在儿童过敏性紫癜的意义  

Significance of Lymphocyte Subgroups and Immunoglobulins in Children with Allergic Purpura

作　　者：林晓燕[1] 周建锋[1] 晁鹏丽[1] 姚弘[1] 金宏伟[1] 

机构地区：[1]厦门大学附属中山医院检验科,福建厦门361004

出　　处：《标记免疫分析与临床》2016年第10期1172-1176,共5页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨外周血淋巴细胞亚群和免疫球蛋白在儿童过敏性紫癜的变化及其临床应用价值。方法选取我院儿科住院并确诊为过敏性紫癜(HSP)的患儿43例为疾病组,选取健康体检儿童60例为健康对照组。分别进行淋巴细胞亚群分析及血清免疫球蛋白检测,应用SPSS19.0统计学软件进行差异显著性检验。结果疾病组治疗前和健康对照组外周血CD3^+、CD3^+CD4^+、CD3^+CD8^+、CD3^+CD4^+/CD3^+CD8^+、CD3-CD(56^+16)^+、CD3-CD19^+和IgG、IgA等指标的差异具有统计学意义(P<0.05)。疾病组治疗前外周血CD3^+、CD3^+CD4^+、CD3^+CD4^+/CD3^+CD8^+水平较健康对照组明显降低,而CD3^+CD8^+、CD3-CD(56^+16)^+、CD3-CD19^+和IgG、IgA水平则明显升高,IgM在两组间差异无统计学意义(P>0.05);治疗后外周血CD3^+、CD3^+CD4^+水平回升,而CD3^+CD8^+水平回落,CD3^+CD4^+/CD3^+CD8^+比值也随之升高;CD3^+CD4^+、CD3^+CD8^+、CD3^+CD4^+/CD3^+CD8^+治疗前后的差异具有统计学意义(P<0.05),而CD3^+、CD3-CD(56^+16)^+及CD3-CD19^+的差异无统计学意义(P>0.05)。另外,免疫功能检测结果显示:IgG和IgA在疾病治疗前后的差异同样具有统计学意义(P<0.05);治疗后IgG和IgA水平明显下降至正常;而IgM在两组间的差异无统计学意义(P>0.05)。结论 CD3^+CD4^+、CD3^+CD8^+、CD3^+CD4^+/CD3^+CD8^+及血清IgG和IgA可作为HSP患儿疾病活动性的实验指标应用于临床,对观察疾病的发生和发展有着重要的意义。临床监测CD3^+CD4^+、CD3^+CD8^+、CD3^+CD4^+/CD3^+CD8^+的变化,对临床症状缓解但机体免疫功能还未完全恢复正常的HSP患儿的后续治疗和随访观察有重要的临床价值。Objective To explore the changes and the clinical value of lymphocyte subgroups and immunoglobulins in children with allergic purpura. Methods We selected 43 children who were diagnosed as allergic purpura （AP） as disease group and 60 healthy children as healthy controls. Lymphocyte subgroup analysis, detection of serum immunoglobulin and statistical analysis with SPSS19.0 were performed. Results CD3^＋, CD3^＋CD4^＋, CD3^＋ CD8^＋, CD3^＋ CD4^＋/CD3^＋ CDS＋, CD3CD（56 ＋ 16）^＋, CD3^-CD19^＋ and IgG, IgA in disease group before treatment were statistically different than the healthy group in （P 〈 0.05 ）. CD3^ ＋ , CD3^ ＋ CD4^ ＋ , CD3^ ＋ CD4^ ＋/CD3^ ＋ CD8 ^＋ levels in disease groups before treatment were significantly lower than healthy controls. CD3 ^＋ CD8 ^＋ , CD3^-CD （ 56 ＋ 16 ） ^＋ , CD3^- CD19 ^＋ , IgA and IgG levels increased significantly. IgM had no statistical difference between the two groups （P 〉 0.05 ）. After treatment CD3^ ＋ , CD3 ^＋ CD4^ ＋ levels were increased, the level of CD3^ ＋ CD8^ ＋ decreased. CD3^ ＋ CD4^ ＋/CD3^ ＋ CD8 ^＋ increased too. There were statistically significant differences in CD3 ^＋ CD4 ^＋ , CD3 ^＋ CD8^ ＋ , CD3 ^＋ CD4 ^＋ / CD3 ^＋ CD8^ ＋ between before and after the treatment（P 〈 0.05 ）. There were statistical differences in CD3 ^＋ , CD3CD （56 ＋ 16） ^＋ and CD3^-CD19^ ＋ （P 〉0.05）. Immune function tests showed： IgG and IgA in disease group also had statistical differences between before and after the treatment（ P 〈 0.05 ）. After treatment IgG and IgA significantly decreased to normal. IgM had no statistical difference between the two groups （ P 〉 0.05）. Conclusion CD3^ ＋ CD4^ ＋, CD3^ ＋ CD8 ^＋, CD3^ ＋ CD4 ^＋/CD3 ^＋ CD8 ^＋, IgG and IgA can be used as activity indexes of AP disease, which can help to observe the occurrence and development of diseases. Clinical monitoring of CD3^ ＋ CD4 ^＋ , CD3^ ＋ CD8 ^＋ , CD3 ^＋ CD4^ ＋ / CD3^ ＋ CD8

关 键 词：过敏性紫癜 淋巴细胞亚群 IGG IGA 

分 类 号：R725.5[医药卫生—儿科]