环糊精/纳米氧化锌-多壁碳纳米管修饰电极的制备及在腺苷测定中的应用  

Preparation of β-Cyclodextrin/Nanometer ZnO-MWNTs Modified Electrodes and their Application in Adenosine Detection

作　　者：赵志伟[1] 申贵隽[1] 谷灵燕 谢澄斌 韩超超[1] 弓晓杰[2] ZHAO Zhi-wei SHEN Gui-jun GU Ling-yan XIE Deng-bin HAN Chao-chao GONG Xiao-jie(College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, China College of Medicine, Dalian University, Dalian 116622, China)

机构地区：[1]大连大学环境与化学工程学院,辽宁大连116622 [2]大连大学医学院,辽宁大连116622

出　　处：《高校化学工程学报》2016年第5期1191-1196,共6页Journal of Chemical Engineering of Chinese Universities

基　　金：国家自然科学基金(81172949)

摘　　要：研究β-CD/纳米ZnO-MWNTs修饰电极的制备与应用,建立了一种测定玛卡与冬虫夏草中腺苷的新方法。腺苷在电极上开路预富集后,用循环伏安法和差分脉冲伏安法研究了腺苷在修饰电极上的电化学行为。结果表明:腺苷在该电极上为两电子两质子的氧化过程,在-4×10~3~4×10^(-1)g×L^(-1)呈现良好的线性关系,检测限可达5×10-4 g×L^(-1)。该方法具有较强的抗干扰能力,在pH=6.0,电位扫描范围-1.2^+1.0 V,扫描速度100 mV×S^(-1)的条件下,加标直接测定冬虫夏草和玛卡的提取液中腺苷的含量,回收率为98%~110%。β-CD/Nanometer ZnO-MWNTs modified electrodes were prepared and a new sensitive electrochemical method for adenosine detection in Ophiocordyceps sinensis and Maca was established. Electrochemical behaviors of adenosine on the modified electrodes were studied by cyclic voltammetry and differential pulse voltammetry after open-circuit preconcentration. The results indicate that the oxidation reaction happens with two-electron and two-proton transfer, which has linear response at the range of 4x10-3-4x10-1 g.L-1 with a detection limit of 5x10-4 g.L-1. Adenosine contents in the extracts of Ophiocordyceps sinensis and Maca can be directly measured under pH=6.0, the potential scan range of -1.2-＋ 1.0V and scanning soeed of 100 mV-s-1, and the method is robust with recovery of 98%-110%.

关 键 词：腺苷 纳米ZNO 循环伏安法 差分脉冲伏安法 

分 类 号：O636.12[理学—高分子化学]