系统性红斑狼疮外周血Tc与Th细胞漂移的变化及意义  

Imbalance of Tc and Th in Peripheral Blood of Patients with Systemic Lupus Erythematosus and Its Significance

作　　者：顾卫军[1] 张庆国[1] 朱伟[1] 郭玉琳[1] 张鲁勤[1] 

机构地区：[1]徐州市第一人民医院血液风湿免疫科,江苏徐州221002

出　　处：《中国实验血液学杂志》2016年第5期1533-1538,共6页Journal of Experimental Hematology

基　　金：徐州市科技计划项目(XZZD1246)

摘　　要：目的:探讨Tc1/Tc2、Th1/Th2及Tc/Th的失衡与系统性红斑狼疮(SLE)分期的关系及意义。方法:采用全血培养、T淋巴细胞荧光标记的流式细胞术分析SLE稳定期和活动期患者及正常健康人外周血中T淋巴细胞亚群及其细胞内因子γ-干扰素(IFN-γ)和白介素4(IL-4)表达率的变化,比较Tc1、Tc2;Th1、Th2,Tc/Th细胞比率在系统性红斑狼疮患者稳定期、活动期的变化,与SLEDAI分期的相关性。结果:SLE活动期患者的CD4^+/CD8^+比值明显低于正常对照组,而其Tc1、Th1细胞表达水平、Tc1/Tc2、Th1/Th2比值则均明显高于SLE稳定期及正常对照组(P<0.05),但在SLE稳定期与正常对照组无差异(P>0.05)。结论:Tc/Th的失衡及Tc与Th细胞向Tc1、Th1漂移的变化在SLE的发病机理中有着重要的作用。Objective：To investigate the imbalance of Tc1/Tc2,Th1/Th2 and Tc/Th in patients with systemic lupus erythematosus（SLE） and its relationship with the clinical stages of SLE.Methods：The full blood culture and flow cytometry with fluorescence-labelled T lymphocytes were used to detect the levels of T4 ymphocyte subsets and its intracellular factors—IFN—γ and IL-4 in peripheral blood from SLP patients in active,inactive stages and normal healthy persons as controls,to compare the changes of Tc1,Tc2;Th1,Th2 levels and Tc/ Th ratio in SLP patients in active and inactive stages,and to analyze their relationship with SLEDAI staging.Results：Compared with healthy controls,the levels of CD4＋ /CD8＋ in active SLE patients were significantly lower,but the levels of Tc1,Th1 in active SLE patients were higher than those in inactive SLE patients and normal controls.The ratio of Tc1 /Tc2 and Th1 /Th2 in the active SLE was higher than that in inactive SLE and normal controls（P〈0.05）,but the difference between inactive SLE patients and controls was not statistically significant（P 〉0.05）.Conclusion：The Tc/Th imbalances and change of Tc and Th cells to Tc1 and Th1 cells play an important role in the pathogenesis of SLE.

关 键 词：系统性红斑狼疮 TC1/TC2 TH1/TH2 细胞因子 

分 类 号：R593.241[医药卫生—内科学]