不同方法检测B群链球菌效果评价  

Diagnostic performance of different methods for detection of group B Streptococcus

作　　者：雷蜜[1] 倪维[2] 

机构地区：[1]湖北省十堰市中医医院检验科,442000 [2]湖北省中医院检验科,武汉430074

出　　处：《检验医学与临床》2016年第20期2847-2849,共3页Laboratory Medicine and Clinic

基　　金：国家自然科学基金资助项目(81573815)

摘　　要：目的分析显色肉汤法、金标免疫层析法（简称金标法）、乳胶凝集法在孕妇B群链球菌（GBS）定植筛查中的应用效果。方法同时采用细菌培养法、显色肉汤法、金标法、乳胶凝集法对1 300例孕35~37周孕妇阴道下端或肛周拭子进行GBS筛查。以细菌培养法作为金标准方法,评价显色肉汤法、金标法、乳胶凝集法的诊断性实验性能。结果显色肉汤法检出率为12.8%、金标法为11.5%、乳胶凝集法为12.1%、细菌培养法为12.0%,不同方法检出率比较差异无统计学意义（P〉0.05）。显色肉汤法准确度、灵敏度最高,分别为98.4%和98.6%;金标法特异度最高,为98.9%,但漏诊率达到14.7%;乳胶凝集法特异度为97.2%,阴性预期值为97.3%。结论与细菌培养法相比,3种方法筛查GBS的检出率相近。显色肉汤法准确、特异,检测系统独立,可作为孕妇GBS定植筛查和辅助诊断的常规方法。金标法检测速度快,可用于门诊大规模常规筛查。乳胶凝集法特异度较好,成本低,适合基层医院开展。Objective To evaluate the diagnostic performance of pigmented broth culture,gold immunochromatographic assay（GICA）and latex agglutination test（LAT）for the detection of group B Streptococcus in pregnant women.Methods Samples from distal vaginal segment and around crissum,collected among 1 300 pregnant women of 35-37 weeks,were screened by the three methods and bacterial culture method simultaneously.Performance of these three methods were evaluated,taking bacterial culture method as standard.Results The detection rates of pigmented broth culture,GICA,LAT and bacterial culture method were12.8%,11.5%,12.1%and 12.0%respectively,which were without significant differences（P〉0.05）.The accuracy and sensitivity of pigmented broth culture were 98.4% and 98.6%,which were the highest.The highest specificity was 98.9% of GICA,but the rate of missed diagnosis of GICA was 14.7%.The specificity of LAT was 97.2%,and it′s negative predictive value was 97.3%.Conclusion Compared with bacterial culture method,the detection rate of the three methods of GBS screening could be similar.Pigmented broth culture method might be accurate,specific,using independent rapid detection system,which could be a routine method for clinical screening and diagnosis of GBS colonization in pregnant women.GICA might be with the characteristics of rapid and convenience,which could be used for large-scale and routine screening in outpatient clinic.LAT might be with fine specificity and low cost,which could be suitable for GBS screening in primary hospital.

关 键 词：B群链球菌 细菌培养法 显色肉汤法 金标法 乳胶凝集法 

分 类 号：R378.12[医药卫生—病原生物学]