慢性应激大鼠外周血促炎细胞因子和肠道微生态的变化  

Changes of pro-inflammatory cytokines in peripheral blood and intestinal microflora in chronic stress rats

作　　者：汪英[1] 李锡春 黄佰彦 张杰[3] 贺莎莎[3] 邓窈窕[3] 罗馨玫[3] 刘杰[3] 王雨晴[3] 

机构地区：[1]四川省人民医院肿瘤科,四川成都610101 [2]中航工业.成飞医院,四川成都610092 [3]四川大学华西医院肿瘤科,四川成都610041

出　　处：《中国临床研究》2016年第10期1303-1306,共4页Chinese Journal of Clinical Research

基　　金：国家自然科学基金(81402402)

摘　　要：目的通过观察慢性应激大鼠外周血促炎细胞因子的表达和肠道微生态的变化,探讨慢性应激对于其体内炎症和肠道微生物的影响。方法对大鼠序贯采用束缚、强迫游泳和噪声三种刺激48 d,分别将进行应激18、33、48 d时存活的大鼠作为慢性应激1组(n=20)、慢性应激2组(n=20)和慢性应激3组(n=20);将未受实验应激原刺激的20只大鼠作为正常对照组。检测并比较4组大鼠一般状况、外周血的促炎细胞因子和肠道微生态状况。结果对照组大鼠的饮食、精神与活动状态良好。3个慢性应激组大鼠均呈现食欲不好、活动减少、毛发无光泽、精神萎靡和对外界刺激反应迟钝,大鼠上述症状、体征随应激时间的延长而明显,即按正常组、慢性应激1、2、3组之序,症状、体征依次加重。随应激程度的递增(正常组→慢性应激1组→2组→3组),大鼠血清白细胞介素(IL)-1β、IL-6、肿瘤坏死因子(TNF)-α和干扰素(INF)-γmRNA相对表达量递升(P均<0.01);大鼠盲肠的肠杆菌和肠球菌定量[集落形成单位(CFU/g)]递升(P均<0.01),乳杆菌和双歧杆菌定量则递降(P均<0.01)。结论慢性应激可显著增加大鼠外周血促炎细胞因子水平和改变肠道微生态,变化程度与应激程度相关。降低促炎细胞因子水平和/或改善肠道微生态状态可能有利于改善其慢性应激状态。Objective To investigate the influence of chronic stress on rat inflammation in vivo and intestinal microorganisms by observing the changes of pro-inflammatory cytokines in peripheral blood and intestinal microflora. Methods By being stimulated with bound,forced swimming and noise of sequential use for 48 days,the surviving rats at 18-,33- and48- day after stress were served as group 1,2,3 of chronic stress（ n = 20 each）,respectively. The rats unprovoked by experimental stressors were served as control group（ n = 20）. The general conditions,pro-inflammatory cytokines in peripheral blood and intestinal microflora conditions of rats in four groups were detected and compared. Results In rats of control group,the diet,mental state and activity were good. In rats of group 1,2 and 3 of chronic stress,poor appetite,decreased activities,dull hair,mental sluggishness and slow response to external stimuli were observed. With the extension of stress time,the aforementioned symptoms and signs were more obvious in turn,namely,the aforementioned symptoms and signs were aggravated in order of control group and group 1,2,3 of chronic stress; the relative expression levels of serum interleukin（ IL）-1β,IL-6,tumor necrosis factor（ TNF）-α and interferon（ INF）-γ mRNA increased in turn（ all P〈0. 01）; the amounts [colony forming unit（ CFU） / g） ] of enterobacteria and enterococcus in rat cecum increased in turn（ all P〈0. 01）,while the amounts of lactobacillus and bifidobacterium in rat cecum decreased in turn（ all P〈0. 01）. Conclusions Chronic stress can significantly increase the pro-inflammatory cytokines in peripheral blood and alter the intestinal microflora,and the degree of change is related to the degree of stress. The decrease of pro-inflammatory cytokines and / or the improvement of the intestinal microflora status will help to improve the state of chronic stress.

关 键 词：慢性应激 大鼠 白细胞介素-1Β 白细胞介素-6 肿瘤坏死因子-α 干扰素-Γ 肠道微生态 

分 类 号：R33[医药卫生—人体生理学]