Survivin及E-cadherin在子宫腺肌病中的表达及相关性  

Expressions and its correlation of Survivin and E-cadherin proteins in adenomyosis

作　　者：夏红蕾[1] 冯颖[2] 安自珍 贾渊雯 谢佳成[1] 

机构地区：[1]兰州市妇幼保健院病理科,甘肃兰州730030 [2]兰州大学第一医院病理科,甘肃兰州730000

出　　处：《中国临床研究》2016年第10期1311-1314,共4页Chinese Journal of Clinical Research

基　　金：兰州市科技计划基金资助项目(2014-1-59)

摘　　要：目的观察凋亡抑制基因生存素(Survivin)和E-钙粘附素(E-cadherin)在子宫腺肌病的表达及其相关性。方法以兰州市妇幼保健院2013年5月至2014年3月68例子宫病变患者为研究对象应用免疫组化En Vision二步法,检测38例子宫腺肌病病灶及在位内膜组织中Survivin及E-cadherin蛋白的表达,并与30例无症状肌瘤患者的正常子宫内膜组织进行对照;应用Western blot法检测Survivin及E-cadherin在子宫腺肌病病灶与正常内膜的表达。统计学分析以α=0.05为检验水准,当采用R×C表χ2检验的分割法时,检验水准调整为α'=0.0125。结果子宫腺肌病病灶组织中,Survivin蛋白的阳性表达率(89.47%)明显高于在位内膜(55.26%)及正常内膜(6.67%,P均<0.0125)。腺肌病病灶组织中E-cadherin蛋白阳性表达率(47.37%)明显低于在位内膜(71.05%)及正常内膜(96.67%,P均<0.0125)。腺肌病病灶中Survivin和E-cadherin表达呈线性负相关(r=-0.988,P<0.01);Western blot结果显示Survivin蛋白在腺肌病病灶的相对表达量明显高于正常内膜,E-cadherin在腺肌病病灶的相对表达量明显低于正常内膜。结论 Survivin及E-cadherin可能与子宫腺肌病的发生、发展有关。Objective To study the expressions of apoptosis-inhibited gene Survivin and E-cadherin in adenomyosis and its correlation. Methods Immunohistochemical EnV ision two step method was used to detect the expressions of Survivin and E-cadherin proteins in ectopic foci and in place endometrial tissues of 38 adenomyosis patients and compared with normal endometrial tissues of 30 asymptomatic myoma patients. Western blot method was used to detect the expressions of Survivin and E-cadherin proteins in ectopic foci and normal endometrial tissues. The criterion for statistical test was difined as α =0. 05,when using R x C table-partitioning chi-square test,the criterion was adjusted for α＇ = 0. 0125. Results The positive expression rate of Survivin protein in ectopic foci of adenomyosis（ 89. 47%） was significantly higher than those in place endometrial tissues of adenomyosis（ 55. 26%） and normal endometrial tissues（ 6. 67%,all P〈0. 0125）. The positive expression rate of E-cadherin protein in ectopic foci of adenomyosis（ 47. 37%） was significantly lower than those in place endometrial tissues of adenomyosis（ 71. 05%） and normal endometrial tissues（ 96. 67%,all P〈0. 0125）. In the foci of adenomyosis,the expressions of Survivin and E-cadherin presented linear negative correlation（ r =- 0. 988,P〈0. 01）. Western blot showed that the relative expression level of Survivin protein in ectopic foci was significantly more than that in normal endometrial tissues,and the relative expression level of E-cadherin protein in ectopic foci was significantly less than that in normal endometrial tissues. Conclusion Survivin and E-cadherin may be related to the occurrence and development of adenomyosis.

关 键 词：子宫腺肌病 生存素 E-钙粘附素 子宫内膜 免疫组化 蛋白印迹法 

分 类 号：R711.74[医药卫生—妇产科学]