人脐带血干细胞移植对2型糖尿病家兔血清学的影响  

Effection of human umbilical cord blood stem cell transplantation on serum of rabbits with type 2 diabetes mellitus

作　　者：丁海霞[1] 王富军[1] 刘贝[1] 史宁[1] 杜亚萍[1] 齐会卿[1] 丁娟[1] 

机构地区：[1]河北医科大学第四医院内分泌科,石家庄050000

出　　处：《中国免疫学杂志》2016年第10期1446-1449,共4页Chinese Journal of Immunology

基　　金：河北省卫生厅课题(No.20150760)

摘　　要：目的:观察人脐带血干细胞(Human umbilical cord blood stem cells,HUCBSC)移植后2型糖尿病家兔血糖、胰岛素及二肽基肽酶Ⅳ(Dipeptidyl peptidase-Ⅳ,DPP-Ⅳ/CD26)的变化。方法:18只家兔随机分成正常对照组(6只,C组)和糖尿病造模组(12只),造模组制备2型糖尿病模型,其中6只经兔耳缘静脉输注给予免疫学表型为CD45^+、CD34^-的HUCBSC移植(A组),6只同法输注等量的PBS液作为对照(B组)。以上三组家兔均喂养观察4周,每天监测家兔血糖,每周测血胰岛素及DPP-Ⅳ/CD26的水平。结果:HUCBSC阳性标志物CD45表达率达100%,阴性标志物CD34表达率为3.5%。与非移植组相比,HUCBSC移植组家兔血糖和DPP-Ⅳ/CD26逐渐下降,胰岛素水平逐渐上升,差异均有统计学意义(P<0.01)。结论:HUCBSC呈圆形或椭圆形贴壁生长,免疫学表型为CD45^+、CD34^-。HUCBSC移植可以明显降低家兔血糖、增加胰岛素分泌量、降低血DPP-Ⅳ/CD26的水平,从而为临床糖尿病及其并发症的治疗提供新的理论依据。Objective： To observe the changes of blood glucose,insulin and dipeptidyl peptidase-Ⅳ（ DPP-Ⅳ/CD26） on type 2diabetes mellitus in rabbits after HUCBSC（ human umbilical cord blood stem cells） transplantation. Methods： 18 rabbits were randomly divided into normal control group（ 6 rats,Group C） and diabetic model group（ 12 rats）. After preparation model of type 2 diabetes,and6 rats of them were treated with HUCBSC（ CD45＋,CD34-） transplantation by ear vein transfusion（ Group A）,and 6 rats were treated with PBS（ Group B）. All three groups of rabbits were fed for 4 weeks,and the blood glucose was monitored every day,and the level of blood insulin and DPP-IV / CD26 were measured every week. Results： The negative expression rate of CD34 in HUCBSC was 96. 5%.The positive expression rate of CD45 in HUCBSC was 100%. Compared with non transplantation group,the blood glucose and DPP-IV /CD26 in the umbilical cord blood stem cell transplantation group were gradually decreased,and insulin level was gradually increased,the difference was statistically significant（ P〈0. 01）. Conclusion： HUCBSC were round or oval,with adherent growth,HUCBSC transplantation can significantly reduce blood glucose,increase insulin secretion,reduce the level of DPP-IV / CD26,the immunological phenotype of HUCBSC was CD45＋,CD34-,thus providing a new theoretical basis for the clinical treatment of diabetes and its complications.

关 键 词：人脐带血干细胞 2型糖尿病 家兔 血清学 

分 类 号：R587.1[医药卫生—内分泌]