三维荧光光谱结合物理和数学分离研究溶解性有机质组成  

Fluorescence Excitation-Emission Matrix Spectra Coupled with Physic and Mathematic Isolation to Study Composition of Dissolved Organic Matter

作　　者：巩学勇[1] 张宏志[1] 侯超[1] 王玉民[1] 董建[1] GONG Xue-Yong ZHANG Hong-Zhi HOU Chao WANG Yu-Min DONG Jian(Institute of Chemical Engineering, Taishan Medical College, Tai＇an 271016, China)

机构地区：[1]泰山医学院化工学院,泰安271016

出　　处：《分析化学》2016年第10期1533-1538,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21501130;31370575);泰安市科技发展计划课题(No.201430774-06)项目资助~~

摘　　要：采用分子排阻色谱和激发/多波段发射荧光检测器,结合三维荧光光谱和平行因子分析,研究了新、老填埋垃圾渗滤液中溶解性有机质(DOM)的组成。实验结果表明,两种渗滤液来源的DOM均含有类蛋白和类腐殖质物质。在新填埋垃圾渗滤液中,类蛋白物质有4种存在形态,包括大分子蛋白质形态、高/低分子量腐殖质结合态和多肽/氨基酸形态;在老填埋垃圾渗滤液DOM中,类蛋白物质只有两种形态,分别为大分子蛋白质形态和腐殖质结合态。相比于分子排阻色谱,三维荧光光谱结合平行因子分析能够分辨出腐殖质和非腐殖质结合态的类蛋白物质,但不能有效区分蛋白质和以多肽/氨基酸形态存在的类蛋白物质。结果表明,三维荧光光谱结合平行因子分析和分子排阻色谱,可以表征DOM中不同形态分布的类蛋白和类腐殖质物质。Combining with fluorescence excitation-emission matrix（ EEM） spectra and parallel factor analysis, size exclusion chromatography（ SEC） equipped with multi-excitation / emission scan model fluorescence detector was used for the analysis of the composition of dissolved organic matter（ DOM） isolated from landfill leachates with different ages. The analytical results showed that the two leachate-derived DOMs both comprised protein- and humic-like substances. However,there were four kinds of protein-like matter in young landfill leachates,i. e.,protein with high molecular weight,protein-like matter bound to humic-like substances with high or low molecular weight,and peptide / amino acids. While there were only two kinds of protein-like matter in old landfill leachates,i. e.,protein with high molecular weight and protein-like matter bound to humic-like substances with high molecule weight. Compared with SEC,EEM spectra coupled with parallel factor analysis could identify the protein-like matter bound with humic-like substances or those presented as non-humic-like substances,though it could not identify the protein-like matter presented as protein and that presented as peptide / amino acids. The experimental results demonstrated that EEM spectra coupled with PARAFAC analysis and SEC could be used to characterize protein- and humic-like matter presented as different species.

关 键 词：溶解性有机质 荧光光谱 分子排阻色谱 平行因子分析 

分 类 号：O657.3[理学—分析化学]