基于分子印迹技术的Ru(bpy)3^2＋/MWCNTs-Nafion-SiO_2纳米粒子电化学发光传感器高效检测17β-雌二醇  

Ru(bpy)_3^(2+)/MWCNTs-Nafion-Silica Nanoparticles Modified Electrochemiluminescent Sensor Based on Molecular Imprinting Technique for Detection of 17β-Estradiol

作　　者：林瑜琳 李小娟[1] 张新颖[1] 曹洁[2,3] 张建华 陈小英[1] LIN Yu-Lin LI Xiao-Juan ZHANG Xin-Ying CAO Jie ZHANG Jian-Hua CHEN Xiao-Ying(College of Environmental and Resource, Fuzhou University, Fuzhou 350116, China AQSIQ Key Laboratory of Drug Detection, Fujian Entry-Exit Inspection and Quarantine Bureau of P. R. C. , Fuzhou 350001, China College of Life Sciences, Fufian Agriculture and Forestry University, Fuzhou 350002, China Fujian Radiation Environmental Supervision Station, Fuzhou 350013, China)

机构地区：[1]福州大学环境与资源学院,福州350116 [2]国家质检总局毒品检测重点实验室,福建国际旅行卫生保健中心,福建出入境检验检疫局,福州350001 [3]福建农林大学生命科学学院,福州350002 [4]福建省辐射环境监督站,福州350013

出　　处：《分析化学》2016年第10期1547-1554,共8页Chinese Journal of Analytical Chemistry

基　　金：福州大学能源与环境光催化国家重点实验室自主课题(No2014C05);福建检验检疫局科技计划项目(Nos FK2013-29,FK2014-01)资助

摘　　要：17β-雌二醇等环境内分泌干扰物在水体中分布广、浓度低,对生态系统及人体危害性大。本研究在金电极表面通过多壁碳纳米管(MWCNTs)的静电吸附作用与Nafion膜的离子交换作用结合纳米二氧化硅(SiO_2),固定化Ru(bpy)_3^(2+),制备Ru(bpy)_3^(2+)/MWCNTs-Nafion-SiO_2修饰电极,提高了修饰电极的灵敏性。通过溶胶-凝胶法制备分子印迹膜提高修饰电极的选择特异性,制得分子印迹-电化学发光传感器(ECL-MIPs)。在优化条件下,即p H 7.4磷酸盐缓冲溶液中,以扫速100 m V/s富集20 min,对17β-雌二醇进行检测,电化学发光强度与17β-雌二醇浓度在0.03~2.00μg/L范围内有良好线性关系,检出限为0.006μg/L。此传感器可用于实际水样中的17β-雌二醇雌二醇的检测,回收率为88.7%~105.0%。Environmental endocrine disruptors such as 17β-estradiol, are widely distributed with low concentration in the water,which has great harm to ecosystems and human health. To improve the detection sensitivity of 17β-estradiol,a Ru（ bpy）3^2＋/ MWCNTs-Nafion-SiO_2 modified electrode was firstly made by electrostatic adsorption of multi-walled carbon nanotubes（ MWCNTs） and ion exchange of Nafion,binding nano-silica and immobilizing Ru（ bpy）3^2＋on the surface of gold electrode. And then a molecular imprintingelectrochemiluminescence sensor（ ECL-MIPs） was acquired by modifying the molecular imprinted membrane with sol-gel method for improving the specific selectivity. Under the optimum conditions（ in 0.1 mol / L PBS,p H 7.4） at a scan rate of 100 m V / s and accumulation time of 20 min）,there was a good linear relationship between the ECL intensity difference and 17β-estradiol concentration in the range of 0. 03- 2 μg / L,with a detection limit of 0. 006 μg / L. The ECL-MIPs sensor was successfully applied to the determination of 17β-estradiol in the water samples with recoveries from 88.7% to 105.0%.

关 键 词：分子印迹 电化学发光 纳米二氧化硅 17Β-雌二醇 

分 类 号：X832[环境科学与工程—环境工程] O657.1[理学—分析化学]