加替沙星磁性表面分子印迹材料的制备及其识别特性的研究  

Preparation and Recognition Characters of Gatifloxacin Magnetic Surface Molecularly Imprinted Polymers

作　　者：赵丽娟[1] 祁玉霞[1] 魏缠玲 李文婧[1] 李亚[1] 王富强[1] 龚波林[1,2] ZHAO Li-Juan QI Yu-Xia WEI Chan-Ling LI Wen-Jing LI Ya WANG Fu-Qiang GONG Bo-Lin(School of Chemistry ＆ Chemical Engineering, Ningxia University, Yinchuan 750021, China Beifang University of Nationalities, Chemical Science and Engineering College, Yinchuan 750021, China)

机构地区：[1]宁夏大学化学化工学院,银川750021 [2]宁北方民族大学化学与化学工程学院,银川750021

出　　处：《分析化学》2016年第10期1562-1567,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.21565001)资助项目~~

摘　　要：以γ-(甲基丙烯酰氧)丙基三甲氧基硅烷(MPS)修饰的磁性二氧化硅(Fe_3O_4@SiO_2)为载体,加替沙星(GTFX)为模板分子,采用表面印迹法制备磁性表面分子印迹聚合物(M-MIPs)。用透射电镜(TEM)及磁化强度分析(VSM)对此聚合物进行了表征。吸附实验和Scatchard分析结果表明,M-MIPs中存在特异性和非特异性两类结合位点。M-MIPs和磁性非印迹聚合物(M-NIPs)对GTFX的最大吸附容量分别为35.1和23.13 mg/g。M-MIPs对于环丙沙星(CPFX)、诺氟沙星(NFLX)、三聚氰胺(MEL)以及四环素(TC)的选择性系数k分别为2.43,5.18,6.61和12.99;M-MIPs相对M-NIPs的相对选择性系数k'分别为2.09,1.95,3.15和2.43,表明M-MIPs对GTFX具有良好的特异性识别能力。将此表面印迹材料用于牛奶中GTFX的分离富集,采用高效液相色谱法检测,回收率大于91.5%。Using gatifloxacin（ GTFX） as template molecule,magnetic surface molecularly imprinted polymers（ M-MIPs） were prepared on the surface of modified magnetic silica（ Fe_3O_4@ SiO_2） with surface molecular imprinting technique. The polymers were characterized by transmission electron microscopy（ TEM） and vibrating sample magnetometer（ VSM）. The static adsorption experiments and Scatchard analysis suggested that there were two types of binding sites in the M-MIPs. The maximum adsorption capacities of M-MIPs and magnetic non-imprinted polymers（ M-NIPs） for GTFX were 35. 1 mg / g and 23. 1 mg / g,respectively. The selectivity coefficients of GTFX M-MIPs to ciprofloxacin（ CPFX）,norfloxacin（ NFLX）,melamine（ MEL）and tetracycline（ TC） were 2.43,5.18,6.61 and 12.99,and the relative selectivity coefficients of M-MIPs to M-NIPs for these four drugs were 2. 09,1. 95,3. 15 and 2. 43,respectively,indicating the good specific recognition capability for GTFX. Combined with high performance liquid chromatographic analysis, the M-MIPs were applied to extract and enrich GTFX in milk sample with the recoveries more than 91.5%.

关 键 词：磁性二氧化硅 加替沙星 表面分子印迹聚合物 吸附 

分 类 号：O631.3[理学—高分子化学]