甲基莲心碱体外抑制人肝癌细胞增殖和侵袭的作用机制研究  

Effect of neferine on proliferation and invasion of human hepatocellular carcinoma cell line HepG2 and Bel-7402

作　　者：周雅君[1,2] 时菁[2] 田耕[2] 周汉新[2] 高国全[1] 

机构地区：[1]中山大学中山医学院,广东广州510000 [2]深圳市第二人民医院中心实验室,广东深圳518000

出　　处：《中国药理学通报》2016年第11期1539-1542,共4页Chinese Pharmacological Bulletin

基　　金：深圳市科技计划项目(No JCYJ20140414170821259);深圳细胞生物技术转化医学重点实验室项目(No ZDSY20130531165409949)

摘　　要：目的探讨甲基莲心碱(neferine,Nef)对人肝癌HepG2和Bel-7402细胞侵袭的影响和作用机制。方法人肝癌HepG2和Bel-7402细胞体外培养,经不同浓度的甲基莲心碱处理后,CCK-8法检测细胞的增殖,Transwell细胞体外侵袭实验观察Nef对细胞侵袭能力的影响;免疫印迹检测Rho相关蛋白的表达。结果 CCK-8结果显示,与对照组比较,甲基莲心碱干预组抑制人肝癌HepG2和Bel-7402细胞增殖,并呈剂量-效应关系(P<0.05);Transwell细胞体外侵袭实验显示,3μmol·L^(-1)甲基莲心碱明显抑制HepG2和Bel-7402细胞的侵袭;免疫印迹结果显示,3μmol·L^(-1)甲基莲心碱作用HepG2和Bel-7402细胞12 h后,RhoA、RhoC和ROCK表达明显降低。结论甲基莲心碱可体外抑制HepG2和Bel-7402细胞的增殖和侵袭,其抑制肝癌细胞的侵袭可能与抑制RhoA、RhoC和ROCK蛋白的表达有关。Aim To investigate the effect of neferine on proliferation and invasion of human hepatocellular carcinoma. Methods HepG2 and Bel-7402 cells were cultured in vitro with different concentrations of neferine,then cell proliferation was observed by CCK-8 assay; cell invasion was observed by transwell invasion assay; the protein expression of RhoA, RhoC and ROCK was detected by Western blot. Results CCK-8results showed that neferine could significantly inhibit cell proliferation in a dose-dependent manner compared with the control group. Transwell invasion assay showed that cell invasion was significantly decreased with neferine 3 μmol · L^(-1). Western blot results showed that RhoA,RhoC and ROCK protein expression was decreased when neferine was co-incubated with hepatocellular carcinoma cells. Conclusion Neferine can inhibit proliferation and invasion of HepG2 and Bel-7402 cells,which is mediated mainly by the inhibition of RhoA,RhoC and ROCK protein expression.

关 键 词：甲基莲心碱 人肝癌细胞HEPG2 人肝癌细胞BEL-7402 增殖 侵袭 RHOA RHOC ROCK 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]