微囊蛋白1及细胞内钙离子在TGF-β1诱导哮喘大鼠气道平滑肌增殖中的作用  

Effect of caveolin- 1 and intracellular Ca^(2+)on the TGF- β 1-induced ASMCs proliferation in asthmatic rats

作　　者：王瑞丽[1] 戴威[1] 李凤琴[2] 吴立琴[2] 戴元荣[2] 

机构地区：[1]温州医科大学附属第二医院,育英儿童医院重症医学科,325027 [2]温州医科大学附属第二医院,育英儿童医院呼吸内科,325027

出　　处：《浙江医学》2016年第20期1634-1636,1642,共4页Zhejiang Medical Journal

基　　金：浙江省医药卫生科技计划(2009A144)

摘　　要：目的探讨微囊蛋白1、细胞内钙离子在转化生长因子β1(TGF-β1)诱导哮喘大鼠气道平滑肌细胞(ASMCs)增殖中的作用。方法离体培养大鼠ASMCs,并分为正常组、哮喘组、TGF-β1干预组、TGF-β1+β-环糊精(β-CD)干预组,CCK-8法检测各组细胞的增殖水平;Western blot法检测各组微囊蛋白1含量;流式细胞仪检测各组ASMCs胞内钙离子荧光强度。结果哮喘组OD值较正常组明显增加(P<0.05),TGF-β1组OD值较正常组、哮喘组明显增加(P<0.05),TGF-β1+β-CD组OD值较TGF-β1组明显增加(P<0.05)。TGF-β1组较正常组和哮喘组微囊蛋白1含量减少(P<0.05),TGF-β1+β-CD组较TGF-β1组微囊蛋白1含量减少(P<0.05)。TGF-β1组较正常组和哮喘组胞内钙离子荧光强度增加(P<0.05),TGF-β1+β-CD组较TGF-β1组胞内钙离子荧光强度增加(P<0.05)。相关关系分析示细胞增殖水平与微囊蛋白1含量呈负相关(r=-0.51,P<0.05),细胞增殖水平与钙离子荧光强度呈正相关(r=0.60,P<0.05),微囊蛋白1含量与钙离子荧光强度呈负相关(r=-0.87,P<0.05)。结论微囊蛋白1可以抑制TGF-β1诱导的ASMCs增殖,这种抑制作用可能部分是通过减少胞内游离钙离子来实现的。Objective To investigate the effect of caveolin-1 and intracellular Ca2＋ （[Ca2＋] （i）） on TGF-β1-inducedproliferation of airway smooth muscle cells（ASMCs） in asthmatic rats. Methods ASMCs of rats were isolated and cultured in vitro.CCK-8 method was used to detect cell proliferation and Western blot method was used to detect the expression of caveolin-1protein. FCMwas used to detect [Ca2＋]（i）. Results OD value in asthma group was higher than that in normal group （P〈0.05）,OD value in TGF-β1 group was higher than that in normal group and asthma group （P〈0.05）, OD value in TGFβ1＋β-CDgroup was higher than that in TGF-β1 group （P〈0.05）. The expression of caveolin-1 in TGF-β1 group was significantly lowerthan that in normal group and asthma group （P〈0.05）, the expression of caveolin-1 in TGF-β1＋ β-CD group was significantlylower than that in TGF-β1 group （P〈0.05）. The [Ca2＋]（i） of ASMCs in TGF-β1 group was higher than that in normal group andasthma group（P〈0.05）, the[Ca2＋]（i） of ASMCs in TGF-β1＋ β-CD group was higher than that in TGF-β1 group（P〈0.05）. Therewas negative correlation between cell proliferation and the expression of caveolin-1 protein （r=-0.51, P〈0.05）, cell proliferationwas positively correlated with the [Ca2＋]（i） （r=0.60, P〈0.05）, caveolin-1 protein was negatively correlated with the [Ca2＋]（i）（r=-0.87, P 〈0.05）. Conclusion Caveolin-1 cab suppress TGF-β1-induced ASMCs proliferation in asthmatic rats, which isassociated with the decrease of [Ca2＋]（i）.

关 键 词：】哮喘 平滑肌细胞 增殖 微囊蛋白 转化生长因子Β1 钙离子 β环糊精类 大鼠 

分 类 号：R562.25[医药卫生—呼吸系统]