NLRP3炎性体相关分子表达与老年2型糖尿病的关系  

Expression of NLRP3 inflammasome-related molecules in elderly patients with type 2 diabetes mellitus

作　　者：洪宝建[1] 苏丽韫[1] 梁丽娟[1] 朱月霞[1] 徐芳[1] 陈建忠[2] 

机构地区：[1]浙江省人民医院检验中心,杭州310014 [2]浙江大学医学院免疫学研究所

出　　处：《浙江医学》2016年第20期1653-1655,共3页Zhejiang Medical Journal

基　　金：浙江省医药卫生科技计划项目(2016KYB032)

摘　　要：目的研究核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)炎性体相关分子表达与老年2型糖尿病(T2DM)的关系。方法采用ELISA法测定30例老年T2DM患者、30例健康体检者(对照组)血浆中IL-1β、IL-18和IL-37水平,分别采用定量聚合酶链反应(PCR)技术和ELISA技术检测糖尿病患者单核细胞来源的巨噬细胞(MDM)表达NLPR3、凋亡相关斑点样蛋白(ASC)和IL-1βm RNA水平及葡萄糖诱导MDM分泌IL-1β和TNF-α的水平。结果老年T2DM患者血浆中的IL-1β和IL-18水平均显著高于对照组,差异均有统计学意义(均P<0.05);而IL-37无统计学差异;T2DM患者MDM中NLPR3、ASC和IL-1βm RNA表达水平均显著高于对照组,差异均有统计学意义(均P<0.05);且经葡萄糖刺激后IL-1β、TNF-α水平显著高于对照组,差异有统计学意义(均P<0.05)。结论 NLRP3炎性体相关分子的表达与炎性体活化可能参与老年T2DM的炎症反应过程。】Objective To investigate the expression of NLRP3 inflammasome-related molecules in elderly patients withtype 2 diabetes mellitus （T2DM） . Methods The plasma levels of IL-1β, IL-18 and IL-37 in 30 T2DM patients and 30 healthycontrols were measured with ELISA; the expression of NLRP3, ASC and IL-1β in monocytes derived macrophages （MDM） weredetected by real time PCR. The contents of IL-1β and TNF-αsecreted by MDMtreated by glucose were also analyzed. ResultsPlasma IL-1β and IL-18 levels in T2DM patients was significantly higher than those in healthy controls （P〈0.05）, while IL-37level was no significantly increased （P〉0.05）. Expression of NLRP3, ASC and IL-1 in MDM and IL-1β, TNF-αsecreted byMDMwere significantly higher than those in controls （P〈0.05）. Conclusion The NLRP3 inflammasome pathway may be involvedin the inflammatory process of elderly patients with T2DM.【

关 键 词：】白细胞介素-1β 2型糖尿病 核苷酸结合寡聚化结构域样受体蛋白3 炎性体 肿瘤坏死因子 

分 类 号：R587.1[医药卫生—内分泌]