不同福利条件下ICR小鼠肝细胞NF-κB表达的比较  

The Expression of NF-κB in Liver of ICR Mice at Different Welfare Level

作　　者：梁磊[1] 马畅[1] 董敏[1] 尤金炜[1] 胡文娟[1] 恽时锋[1] 

机构地区：[1]南京军区南京总医院比较医学科,全军实验动物科普与伦理教育基地,全国科普教育基地,南京210002

出　　处：《实验动物科学》2016年第4期1-5,共5页Laboratory Animal Science

摘　　要：目的比较不同福利水平下ICR小鼠肝脏NF-κB的表达。方法选取6周龄雄性ICR小鼠,交叉使用制动和丰富环境干预形成不同的福利环境,观测小鼠的体质量增重、饲料能效及脾脏系数,ELISA法测定血清IL-2和CORT,实时定量PCR和免疫印迹法分别检测小鼠肝细胞内NF-κB p65的mRNA和蛋白表达水平。结果与对照组相比,丰富环境组小鼠的体质量增重显著提高(P<0.05),其他指标无差异(P>0.05);丰富环境+制动组小鼠的体质量增重、脾脏系数显著低于对照组(P<0.05),而CORT、NF-κB p65的基因和蛋白表达显著高于对照组(P<0.05);制动组的体质量增重、饲料能效、脾脏系数及血清IL-2均显著低于对照组(P<0.05),CORT、NF-κB p65的基因和蛋白表达均显著高于对照组(P<0.05)。其中制动组的体质量增重显著低于丰富环境+制动组(P<0.05),CORT、NF-κB p65的基因和蛋白表达显著高于丰富环境+制动组(P<0.05)。结论不同组别小鼠存在福利水平差异,NF-κB表达差异与福利差异相类似,福利水平与NF-κB表达水平之间存在紧密关联。Objective To compare the expression of NF-κB in liver of ICR mice at different welfare level. Method ICR mouse , 6 years old , welfare were infected by both restraint stress and environmental enrichment , or only one of them. Body weight gain and food consumption were recorded throughout the study , IL-2 and CORT in blood were detected by ELISA , mRNA and protein expression of NF-κB p65 in liver were detected after the study finished by real-time quantitative PCR and Western blotting. Result Compared with group C（control） , the body weight gain of group E （environmental enrichment） was higher （P 〈 0.05）. The body weight gain of group ER （environmental enrichment and restraint） was decreased （P 〈 0.05）. CORT , mRNA and protein expression of NF-κB p65 were higher （P 〈0.05）. The body weight gain , FER and IL-2 of group R（ restraint） were decreased （P 〈 0.05）. CORT , mRNA and protein expression of NF-κB p65 were higher （P 〈 0.05）. Compared with group ER , the body weight gain of group R （restraint） were decreased （P 〈 0.05）. CORT , mRNA and protein expression of NF-κB p65 were higher （P 〈 0.05）. Conclusion Difference of welfare did exist , and the difference of expression of NF-κB p65 were similar to it. There were relations between those two ones.

关 键 词：动物福利 NF-ΚB 基因表达 

分 类 号：Q813[生物学—生物工程]