红景天苷对压力负荷型慢性心衰大鼠心脏的作用及机制  

The Effect and Mechanism of Salidroside in Pressure-overload Chronicity Heart Failure Rats

作　　者：王晨阳[1] 曹雪滨[2] 曹海涛[1] 徐鹏[2] 

机构地区：[1]解放军第252医院动物实验室,保定071000 [2]解放军第252医院心内科,保定071000

出　　处：《实验动物科学》2016年第4期6-10,共5页Laboratory Animal Science

摘　　要：目的研究红景天苷对压力负荷型慢性心衰大鼠心电图的作用及可能的机制。方法 30只雄性SD大鼠随机分成假手术组(S组)、心衰组(HF组)、红景天苷组(SAL组),每组10只大鼠。HF组、SAL组采用腹主动脉缩窄法制备模型,S组只穿线不缩窄。SAL组术后12周给予红景天苷皮下注射,60 mg/kg·d,共30 d。假手术组和心衰组给予同等剂量的生理盐水。给药30 d后,Ⅱ导联心电图检测肢体导联并统计分析数据。将大鼠麻醉后,腹主动脉抽血离心后取血清,按试剂盒方法测定超氧化物歧化酶(SOD)、B型钠尿肽(BNP)、丙二醛(MDA)。结果术后12周,心电图检测显示几个特点:SAL组和HF组比较:1)心率和P波振幅有明显降低(P<0.05);2)R波振幅明显减小(P<0.05);3)心电图的J点位移明显降低(P<0.05),而ST高度则明显抬高(P<0.05)。血清酶的检测结果发现,和心衰组相比,SAL组大鼠血清中的SOD值明显增加(P<0.05),BNP值则明显降低(P<0.05)。结论红景天苷可以改善心衰大鼠的心功能,减轻心肌细胞的损伤,其机制可能与抗氧化作用相关。Objective To explore the effect and mechanism of Salidroside in pressure-overload chronic heart failure rats. Method Totally 30 SD rats were randomly divided into sham operation group, heart failure group and Salidroside group, 10 rats in each group. The HF group and SAL group were prepared by abdominal aorta constriction method, and S group only was worn line not constricted. SAL group was given subcutaneous injection from 12 weeks after operation by 60mg/ kg d in 30 days. The S group and HF group received the same dose of normal saline. After 30 days, two lead ECG parameters of limb a was measured and statistically analyzed. After the end of intervention, the rats were anesthetized, and abdominal aortic blood taken from the upper serum after centrifugation. Kit method was used to measure superoxide dismutase （SOD） , brain natriuretic peptide （BNP） , malondialdehyde （MDA）. Result Twelve weeks after operation, there was a decrease in heart rate, P amplitude and R amplitude comparing by those of the heart failure group （ P 〈 0.05 ）. ECG showed a significant and ubiquitous J point depression （P 〈 0.05）, with ST segment notable elevation （P 〈 O. 05）. SOD activity was significantly increased and BNP concentration was significantly decreased in SAL group compared to HF group （P 〈0.05）. Conclusion These results show that Salidroside protects pressure-overload rat heart from failure possibly through reducing oxidation.

关 键 词：红景天苷 压力超负荷 心衰 心电图 

分 类 号：R285.5[医药卫生—中药学] R332[医药卫生—中医学]