红藻氨酸致癫痫大鼠不同时点神经生长因子变化研究  

The Change of Nerve Growth Factor Genes Expression at Different Time of Kainic Acid-induced Epilepsy in Rats

作　　者：王红义[1] 冯小明[1] 郭晓宇[1] 陆璐[1] 申健[1] 肖攀[1] 牛廷献[1] 

机构地区：[1]兰州军区兰州总医院动物实验科,兰州730050

出　　处：《实验动物科学》2016年第4期37-40,47,共5页Laboratory Animal Science

基　　金：甘肃省自然科学基金(No.1107RJZA269)

摘　　要：目的探讨红藻氨酸（KainicAcid，KA）致癫痫大鼠癫痫发作过程中海马组织神经生长因子（nervegrowthfactor，NGF）不同时间点表达量的变化。方法腹腔注射KA建立大鼠癫痫模型。采用TaqMan探针实时定量PCR（Real—timequantitativePCR）技术，检测注射KA后不同时点大鼠海马组织中NGF表达量的变化。结果与NGF表达量与生理盐水对照组（Ns）相比，6—12hNGF表达量明显低于Ns组（P〈0．05）、48—72hNGF表达量开始升高并高于NS组，在24、72h时其表达量显著高于NS组（P〈0．05）。结论NGF对致癫大鼠的海马具有修复与保护作用。Objective To investigate the difference of expression of nerve growth factor（NGF） at different time by rats that induced epilepsy by kainic acid （ KA）. Method The epilepsy model was used kainic acid intraperitoneal injectioned in rat. The nerve growth factor expression were detected by real-time fluorescent quantitative PCR assay with TaqMan probe in different time after intraperitoneal KA. ResuR Compared with normal sodium（NS） group, the expression of NGF were lower at 6-12 h , and were higher at 48 h and 72 h（P 〈0.05） ,but the expression of NGF were risied up at 48-72 h and higher by NS. Conclusion NGF have reparation and protection the hippocampus of rats that induced epilepsy by KA.

关 键 词：癫痫 定量PCR 神经生长因子 发作程度 修复 

分 类 号：R33[医药卫生—人体生理学]