桃核承气汤对肝性脑病大鼠肠道菌群的影响分析  

The effect of Taohechengqi Decoction on intestinal flora in the hepatic encephalopathy in rats model

作　　者：李瑞生[1] 李晓娟[1] 王仲霞[2] 王立福[2] 杜宁[2] 朱云[2] 孙永强[2] 景婧[2] 王丽苹[2] 王睿林[2] 张萍[2] 

机构地区：[1]解放军第302医院临床研究管理中心,北京100039 [2]解放军第302医院中西医结合诊疗与研究中心,北京100039

出　　处：《实验动物科学》2016年第4期63-66,共4页Laboratory Animal Science

基　　金：国家"十二五"科技重大专项资助项目(No.2012zx10005010-002-002)

摘　　要：目的研究桃核承气汤对肝性脑病大鼠肠道菌群是否存在相关的影响。方法本实验对40只SD大鼠采用腹腔注射硫代乙酰胺(TAA)和口腔灌胃该中药方剂汤的方法,观察大鼠的神经HE分级和血氨的作用,以及对肠道菌群的影响。结果各剂量组不同程度地可降低大鼠HE级别和血氨的浓度,空肠和回肠的细菌计数与模型组相比呈下降趋势且P<0.05,对空回肠菌群具有显著的回调作用。同时各剂量组的双歧杆菌和大肠杆菌也出现回调和B/E值增高的现象。结论该方剂汤对肝性脑病大鼠的肠道菌群具有正向的影响作用,可增加胃肠道的定植抗力作用。Objective To study the influences of Taohe chengqi decoction on intestinal flora in the rats model with hepatic encephalopathy （HE） induced by TAA. Method In this experiment, 40 SD rats were injected intraperitoneally with TAA and was garaged with the traditional Chinese medicine decoction, then the effects of Taohe chengqidecoetion were observed on neurology test,blood ammonia and intestinal flora. Result Every dose of Taohe ehengqi decoction significantly decreased the staging of HE and blood ammonia. Compared with the model group,the bacteria/ count of the jejunum and ileum of the traditional Chinese medicine showed a decreasing trend （P 〈 0.05） , and Taohe chengqi decoction has a callback effect on the bacterial of the jejunum and ileum. At the same time,there still has a callback effect on Bacillus bifidus and Escherichia coli in the colon in each dose group of traditional Chinese medicine,then Both B/E value increased. Conclusion The decoction has a positive effect on the intestinal flora of rats with hepatic encephalopathy, which can increase the colonization resistance of gastrointestinal tract.

关 键 词：桃核承气汤 大鼠 肠道菌群 

分 类 号：R332[医药卫生—人体生理学]