玉米对大斑病菌抗性中防卫基因的RT-PCR研究  

RT-PCR ANALYSIS OF DEFENCE GENES INVOLVED IN RESISTANCE TO Exserohilum turcicum IN MAIZE

作　　者：尚俊军[1] 余潮[1] 邹燕[1] 朱友林[1] 

机构地区：[1]南昌大学生物科学工程系,江西南昌330047

出　　处：《南昌大学学报（理科版）》2002年第2期138-142,共5页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目 (3976 0 0 4 4 );江西省自然科学基金资助项目 (9930 0 4 1)

摘　　要：以玉米抗、感大斑突脐孢菌近等基因系为材料 ,采用PCR和RT -PCR技术 ,初步研究了苯丙氨酸解氨酶基因 (PAL)、胞液抗坏血酸过氧化物酶基因 (apx)和过氧化物酶K基因 (POXK)等防卫基因与玉米对大斑病菌抗性的关系。结果表明 ,在玉米抗病和感病自交系间 ,没有发现防卫基因在结构和组成上的差异。但在受到玉米大斑突脐胞菌侵染诱导后 ,玉米抗病系中防卫基因在转录水平上的表达强度明显高于感病系。Relationship of phenylalanine ammonia lyase gene (PAL), cytosolic ascorbate peroxidase gene (apx) and peroxidase K gene (POX K) with resistance to Exserohilum turcicum in maize was analysed by PCR and RT-PCR, in which resistant and susceptible isolines of maize were used as experimental materials.It was found that there was no difference in the structure and constitution of defense genes between resistant and susceptible isolines, but the transcriptional expression of these three defense genes in resistant isoline was obviously higher than in susceptible isoline after inoculation with E.turcicum.

关 键 词：大斑病菌 抗性 玉米大斑病 防卫基因 RT-PCR 抗病分子机制 抗病基因工程 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治] Q943.2[农业科学—植物保护]