耐力训练对2型糖尿病大鼠骨骼肌leptin-AMPK-ACC信号通路的影响  

Effects of Endurance Training on Leptin-AMPK-ACC Pathway in Type 2 Diabetic Rats Skeletal Muscles

作　　者：衣雪洁[1] 常波[1] 马铁[1] 高海宁[1] 赵大林[1] 李雨[1] 曹师承[2] 

机构地区：[1]沈阳体育学院运动人体科学学院,辽宁沈阳110102 [2]中国医科大学运动医学教研室,辽宁沈阳110013

出　　处：《沈阳体育学院学报》2016年第4期88-93,共6页Journal of Shenyang Sport University

基　　金：国家自然科学基金(30971414);辽宁省优秀人才支持计划项目(WR2013015);沈阳体育学院重点学科建设项目(XKFX1511)

摘　　要：目的:探讨长期有氧运动对糖尿病大鼠骨骼肌瘦素(leptin)-腺苷酸激活蛋白激酶(Adenine monophosphate activated protein kinase,AMPK)-乙酰辅酶A羧化酶(Acetyl-Co A carboxylase,ACC)通路和糖脂代谢紊乱的影响,为揭示运动防治糖尿病的机制提供依据。方法:采用高脂饮食结合小剂量链脲佐菌素(Streptozotocin,STZ)建立2型糖尿病大鼠模型。将其分成对照组(CON)、糖尿病组(AC)、糖尿病运动组(AE),运动组采用6周游泳训练,1 h/d,5d/周。结果:1 6周运动干预后,AC组大鼠出现高体重、高血糖、高胰岛素和高瘦素现象;同时骨骼肌中离脂肪酸(free fatty acid,FFA)和甘油三酯(Triglyceride,TG)均明显高于NC组,肌糖原(muscle glycogen,MG))含量却显著下降;并伴有骨骼肌瘦素含量明显升高(P<0.01),瘦素受体、AMPKα1、α2的磷酸化水平与蛋白表达显著降低(P<0.05或P<0.01);ACC磷酸化水平显著减少(P<0.05)。26周运动游泳训练有效降低了"四高现象";骨骼肌中的TG和FAA含量也显著下降,肌糖原含量显著增加(P<0.05或P<0.01);瘦素受体、AMPKα1、α2的磷酸化水平与蛋白表达,以及ACC的磷酸化水平均明显增加(P<0.05或P<0.01)。结论:2型糖尿病大鼠骨骼肌leptin-AMPK-ACC信号转导通路受损,引起骨骼肌糖脂储备的异常,可能是引发机体胰岛素抵抗和糖脂代谢异常的原因之一;长期有氧耐力训练可部分修复骨骼肌leptin-AMPK-ACC信号转导通路的受损,改善胰岛素抵抗和糖脂代谢异常。Objective： To explore effects of long-term aerobic training on leptin-AMPK（ Adenine monophosphate activated protein kinase）-ACC（ Acetyl-Co A carboxylase） pathways in skeletal muscles and disorder of glucose-lipid metabolism of diabetic rats and to eveal the mechanism of exercise on diabetes prevention. Methods： SD male Rats were divided into 3groups by body weight randomly： control group（ Con）,diabetes group（ AC）,diabetes exercise group（ AE）. Rats of AC group were administered with streptozocin（ STZ） and hyper-lipid diet to establish type 2 diabetes model. Rats of AE group were established as type 2 diabetes model as those AC group,and then did swimming training 6 w eeks（ 1 hour per day,5days per week）. Results：（ 1） After 6 weeks of training,AC group compared with Con group. Rats of AC group had high weight,high blood glucose,hyperinsulinism and hyperleptinemia; Triglyceride（ TG）,free fatty acid（ FFT） and leptin in skeletal muscles increased significantly（ P 0. 01）; muscle glycogen（ MG）,and phosphorylation level of AM PKα2 in skeletal muscles decreased significantly（ P 0. 01）; leptin receptor,phosphorylation level of AM PKα1 and ACC in skeletal muscle decreased significantly（ P 0. 05）.（ 2） After 6 weeks of training,AE group was compared with AC group. The tendency of high weight,high blood glucose,hyperinsulinism and hyperleptinemia was decreased by exercise; TG in skeletal muscles decreased significantly,while MG increased significantly（ P 0. 01）; FFA in skeletal muscles decreased significantly（ P 0. 05）; leptin receptor,phosphorylation level of AM PKα1 / α2 and ACC in skeletal muscle increased significantly（ P 0. 05）. Conclusion： Injury of leptin-AM PK-ACC pathway induces glycogen and lipid reserves disorder in skeletal muscles of type 2 diabetic rats. This may be a reason of insulin resistance and glucose-lipid metabolism disorder in type2 diabetic rats body. Long-term aerobic endurance training can improve leptin-AM PK-ACC p

关 键 词：糖尿病 骨骼肌 瘦素 AMPK 脂代谢 

分 类 号：G804.2[文化科学—运动人体科学]