移植时间和检测方法与allo-HSCT受者的DNA嵌合率及法医学意义  

Transplantation time and detection method of allo-HSCT and the DNA of the recipients and its significance in forensic medicine

作　　者：丁春丽[1,2] 赵峰[1,2] 周雪 李学博[1,2] 孟浩[2] 房强[2] Ding Chunli Zhao Feng Zhou Xue Li Xuebo Meng Hao Fang Qiang(Shandong University Key Laboratory for Evidence Identification Judicial identification center of Shandong University of Political Science and Law, Jinan 250014, China Institute of Criminal Science and Technology, Zhoushan Public Security Bureau, Zhejiang Zhoushan 316021, China)

机构地区：[1]山东省高校证据鉴识重点实验室,山东济南250014 [2]山东政法学院司法鉴定中心,山东济南250014 [3]舟山市公安局刑事科学技术研究所,浙江舟山316021

出　　处：《中国法医学杂志》2016年第5期459-462,共4页Chinese Journal of Forensic Medicine

基　　金：山东政法学院科研计划项目(2014F14B);山东省自然科学基金资助项目(2R2014HQ018)

摘　　要：影响allo-HSCT受者DNA嵌合率的因素有很多:如受者的疾病类型、移植前预处理方案、移植时病情的严重程度、造血干细胞来源、局部的GVHD、HLA是否相合等。本文主要讨论与法医学关系最为密切的2个影响因素,即移植后时间和检测方法的灵敏度。法医物证学工作者在检案实践中,对allo-HSCT受者进行亲权鉴定或个体识别时,需注意移植后时间和检测方法的灵敏度对嵌合率的影响。女性受者接受男性供者HSCT的情况下,受者毛囊中可以检出供者Y染色体DNA,检案实践中来源于犯罪现场的生物检材不能仅仅检测Y染色体特异遗传标记,以免导致错误判断。There were many factors that affect the chimerism of donor DNA, such as the type of disease, the treatment of the disease, the severity of the disease, the source of hematopoietic stem cells, HLA, GVHD, and so on. This paper mainly discussed the two factors that affect forensic science, including the posttransplantation intervals and sensitivity of the detection method. Forensic workers in forensic practice and paternity and individual identification of allo HSCT recipients, should pay attention to the time after transplantation and the detection method of sensitivity of chimerism. When female recipients received HSCT in the case of male donors, Y chromosome DNA of the donor can be detected from the recipient＇s hair follicles. The case of biological specimens from the crime scene can not only detect Y chromosome specific genetic markers, so as to avoid wrong identification.

关 键 词：法医物证学 ALLO-HSCT 嵌合率 移植时间 检测方法 

分 类 号：D919[医药卫生—法医学]