4个多拷贝RM Y-STR基因座的异常分型  

Anomalous genotype observation of 4 multi-copy RM Y-STRs

作　　者：张文琼[1,2] 肖超[1] 余谨[3] 贾雲舒 黄代新[1] Zhang Wenqiong Xiao Chao Yu Jin Jia Yunshu Huang Daixin(Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China Qingdao Licang district people＇s procuratorate, Shandong Qingdao 266100, China Wuhan blood center, Wuhan 430030, China)

机构地区：[1]华中科技大学同济医学院法医学系,湖北武汉430030 [2]青岛市李沧区人民检察院,山东青岛266100 [3]武汉血液中心,湖北武汉430030

出　　处：《中国法医学杂志》2016年第5期476-479,共4页Chinese Journal of Forensic Medicine

摘　　要：目的观察湖北汉族人群中4个多拷贝RM Y-STR基因座的异常分型。方法使用文献报道及自行设计的荧光标记引物对252个无关男性个体样本进行扩增,并用AB 3130遗传分析仪对扩增产物进行检测分析。结果在所有样本中共发现25例异常多等位基因分型,其中基因座DYF387S1出现5例,DYF399S1出现15例,DYF403S1出现1例,DYF404S1出现4例。25例异常分型存在于20个样本中,发生率为7.94%,其中4个样本在两个以上基因座上均出现异常分型。结论多拷贝RM Y-STR基因座异常分型发生率较高,在法医学的实际应用中应予以注意。Objective To observe anomalous genotypes of 4 multi-copy RM Y-STRs in Han population in Hubei province. Methods 252 unrelated male samples were amplified using reported and newly designed primers, then detected and analyzed by AB 3130 genetic analyzer. Results A total of 25 anomalous multi-band patterns were observed in 20 samples corresponding to an incident rate of 7.94%. 5 anomalous genotypes were observed in DYF387S1 locus, 15 in DYF399S1, 1 in DYF403S1 and 4 in DYF404S1. Four samples showed extra alleles in more than one locus. Conclusion Anomalous genotype has high incident rates in RM Y-STR markers and requires extensive attention in forensic practice.

关 键 词：法医物证学 Y-STR 快速突变 异常基因型 

分 类 号：D919[医药卫生—法医学]