高效絮凝菌A9在不同碳源培养条件下差异表达蛋白鉴定及分析  

Mass spectrometry identification of differential expression proteins from an efficient bioflocculant-producing strain A9 under different carbon source culture conditions

作　　者：刘金亮[1] 姜彬慧[1] 赵鑫[1] 胡筱敏[1] 杨程程[1] 李凤达 马云峰[3] 

机构地区：[1]东北大学资源与土木工程学院,沈阳110819 [2]北京桑德环境工程有限公司,北京101102 [3]沈阳航空航天大学能源与环境学院,沈阳110316

出　　处：《生态学杂志》2016年第11期2999-3004,共6页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(51278090);中央高校基本科研业务费专项资金项目(N120601003)资助

摘　　要：研究了高效絮凝菌A9在普通培养基、葡萄糖培养基和甘露糖培养基的培养条件下蛋白质组表达的差异。从这3种不同培养基培养的菌体中提取蛋白,进行蛋白质双向电泳,胶图分析后选取差异蛋白进行质谱鉴定。胶图分析结果表明:絮凝菌A9在不同碳源培养条件下蛋白质表达有较大差异。通过质谱分析,共有54个蛋白得到成功鉴定,包括二氢硫辛酸脱氢酶、磷酸丙糖异构酶、果糖-1,6-二磷酸酶、甘油醛-3-磷酸脱氢酶、磷酸甘油酸激酶、顺乌头酸酶、磷酸酰基转移酶、肽基脯氨酸顺反异构酶、延伸因子G等。这些差异蛋白的功能主要与能量代谢、糖类合成和代谢、脂类运输和代谢、蛋白翻译后修饰以及核糖体结构和生成有关。本文从蛋白水平阐明了絮凝菌A9在不同碳源培养条件下部分蛋白表达差异,为优化菌株絮凝剂培养条件提供了依据。To study the differential proteome expression of an efficient bioflocculant-producing strain A9 cultured respectively in ordinary broth medium, dextrose medium and mannose medium, proteins were compared and analyzed by twodimensional electrophoresis （2-DE） and identified by mass spectrometry. The results showed significant differences of proteome expression among test groups cultured with different carbon sources. There were 54 differential proteins identified by mass spectrometry, including dihydrolipoyl dehydrogenase, triosephosphate isomerase, fructose-1,6-bisphosphatase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, aconitase, phosphate acyltransferase, peptidylprolylcistrans isomerase, elongation factor G, etc, which were mainly related to synthesis and metabolism of carbohydrates, transportation and metabolism of lipids, posttranslational modification of proteins, and structure and generation of ribosomes. The proteomic research of bioflocculant-producing strain A9 in different media would provide theoretical basis for optimizing culture conditions of strain A9.

关 键 词：絮凝剂 双向电泳 差异表达蛋白 质谱分析 

分 类 号：Q93[生物学—微生物学] Q78