A 1,8-naphthalimide-derived turn-on fluorescent probe for imaging lysosomal nitric oxide in living cells  

作　　者：Wei Feng Qing-Long Qiao Shuang Leng Lu Miao Wen-Ting Yin Li-Qiu Wang Zhao-Chao Xu 

机构地区：[1]College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China [2]State Key Laboratory of Fine Chemicals, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China [3]State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China

出　　处：《Chinese Chemical Letters》2016年第9期1554-1558,共5页中国化学快报（英文版）

基　　金：financial supports from the National Natural Science Foundation of China (Nos. 21276251, 21506206, 21402191, 21502189);the 100 talents program funded by Chinese Academy of Sciences, Dalian Cultivation Fund for Distinguished Young Scholars (Nos. 2014J11JH130, 2015J12JH205);the National Science Fund for Excellent Young Scholars (No. 21422606)

摘　　要：Nitric oxide has played an important role in many physiological and pathological processes as a kind of important gas signal molecules. In this work, a new fluorescent probe LysoNO-Naph for detecting NO in lysosomes based on 1,8-naphthalimide was reported. LysoNO-Naph has sub-groups of o-phenylene- diamine as a NO reaction site and 4-（2-aminoethyl）-morpholine as a lysosome-targetable group. This probe exhibited good selectivity and high sensitivity （4.57 μmol/L） toward NO in a wide pH range from 4 to 12. Furthermore, LysoNO-Naph can be used for imaging NO in lysosomes in living cells.Nitric oxide has played an important role in many physiological and pathological processes as a kind of important gas signal molecules. In this work, a new fluorescent probe LysoNO-Naph for detecting NO in lysosomes based on 1,8-naphthalimide was reported. LysoNO-Naph has sub-groups of o-phenylene- diamine as a NO reaction site and 4-（2-aminoethyl）-morpholine as a lysosome-targetable group. This probe exhibited good selectivity and high sensitivity （4.57 μmol/L） toward NO in a wide pH range from 4 to 12. Furthermore, LysoNO-Naph can be used for imaging NO in lysosomes in living cells.

关 键 词：Fluorescent probe Nitric oxide Lysosome localized Imaging in living cells 

分 类 号：O657.3[理学—分析化学]