急性淋巴细胞白血病单链抗体(scFv)的筛选与鉴定  

Screening and Characterization of Human Phage Antibody to Permethrin

作　　者：马巍娜[1] 刘雪林[2] 宋宏彬[2] 沈建良[1] 黄友章[1] 刘毅[1] 向丹[1] 

机构地区：[1]中国人民解放军海军总医院血液科,北京100037 [2]中国人民解放军军事医学科学院十所,北京100039

出　　处：《现代检验医学杂志》2016年第5期46-49,共4页Journal of Modern Laboratory Medicine

摘　　要：目的：筛选急性淋巴细胞白血病病人单链可变区 scFv抗体，为进一步表达并得到其特异性强的抗体片段创造条件。方法实验利用初诊急性淋巴细胞性白血病病人血清为包被抗原，采用噬菌体表面展示技术，从半合成的人源噬菌体抗体库中筛选其特异性噬菌体抗体，首先把靶抗原包被于免疫平板后，加入噬菌体抗体库，这样能与靶抗原特异性结合的噬菌抗体就被固定在免疫平板上，不能特异结合的噬菌体则被漂洗掉；将特异结合的噬菌体洗脱下来，侵染大肠埃希菌，就可以得到含特异抗体基因的噬菌粒。结果经过3轮“吸附-洗脱-扩增”筛选过程，获得抗原特异性较强的白血病病人可变区噬菌体抗体片段并鉴定。结论获得了一株亲和性较好的抗体片段，为下一步片段表达、鉴定及临床应用研究创造了条件。Objective To do screening acute lymphoblastic leukemia patients scFv antibody single chain variable region to cre-ate conditions for the expression and obtain further specificity of antibody fragments.Methods In this study,patients with newly diagnosed acute lymphoblastic leukemia serum as coating antigen using phage display technology,screening phage an-tibody specificity from the semi-synthetic human phage antibody libraries,the first to target the immune antigen-coated tab-let,phage library was added,so that with the target antigen-specific binding phage antibody was immobilized on plates immu-nization,could not be specifically bound phages were rinsed.The eluted specific binding phage,E.coli infection.Could get the specific antibody gene containing phagemid.Results After three “adsorption-elution-amplification”screening process,got stronger leukemia patient antigen-specific phage antibody variable region fragment and identification.Conclusion Got better strain affinity antibody fragments,to create the conditions for the next fragment expression,identification and clinical appli-cation.

关 键 词：抗体 噬菌体表面展示技术 急淋白血病 SCFV 

分 类 号：R557.4[医药卫生—血液循环系统疾病] R392.11[医药卫生—内科学]