人类表皮生长因子受体2在乳腺癌表达与临床的相关病理特征分析  

Expression of Human Epidermal Growth Factor Receptor 2 and Correlation with Clinical Pathological Features in Breast Cancer

作　　者：刘延梅[1] 马少君[1] 张月浪[1] 康晓丽[1] 梁晓燕[1] 周理乾[1] 李文生[1] 

机构地区：[1]陕西省人民医院西院二病区

出　　处：《现代检验医学杂志》2016年第5期91-93,96,共4页Journal of Modern Laboratory Medicine

基　　金：陕西社发科技攻关项目(编号:2016SF-168);陕西科学技术研究发展计划项目(编号:2013K-12-03-19)

摘　　要：目的：评估人类表皮生长因子受体2（HER2）预测价值，并分析其与常见的组织病理学参数的相关性。方法收集陕西省人民医院2011年～2014年之间160例接受手术治疗的乳腺癌患者组织标本，通过免疫组织化学方法（IHC）和荧光原位杂交（FISH）检测 HER2水平，通过χ^2检验用来评估 HER2基因扩增状况及不同临床病理特性的相关性，临床参数包括：肿瘤大小、组织学分级、雌激素受体（ER）和孕激素受体（PR）表达，年龄、绝经情况和 Ki-67指数。结果 HER-2表达与组织学分级、淋巴结转移、ER水平、PR，Ki-67指数差异存在统计学意义（均P＜0.05）。相对于 HER-2＋组患者， HER-2－病变多表现为雌激素 ER阴性、孕激素阴性、ER阴性、淋巴结阴性、ki-67≥20％。结论 HER-2表达与多种临床病理因素存在相关性。Objective To evaluate of value of HER2 as a prognostic marker,and to analysis associations with common his-topathological parameters in BC cases.Methods In this study,formalin-fixed,paraffin-embedded samples of tumors from 160 breast cancer patients who underwent curative surgery proce-dures between 2011 and 2014 were tested by immunohisto-chemistry （IHC）as aprimary estimate of HER2 status,followed by fluorescence in situ hybridization （FISH）.Concordance rate between IHC and FISH was evaluated.Theχ^2 test was used to evaluate the correlation between HER2 gene amplifica-tion status and different clinical pathological features including：（estrogen receptor）ER and （progesterone receptor）PR ex-pression,age,menopausal status and tumor size.Results There was a significant inverse association between expression of hormone receptors （ER and PR）and HER2 amplification （all P〈0.05）among the patients studied.However,no relation-ship was observed between HER2 amplification and age,menopausal status and tumor size （P〉0.05）.HER2-negative le-sions were of higher grade and more likely to be ER-negative,PR-negative,p5 3-positive,lymph node metastasis,and also Ki-67≥20% as compared to the HER2-positive group and HER2 overe xpression was associated with the levels of ER and PR. Conclusion There is correlation of many clinical factors with HER2 expression.

关 键 词：乳腺癌 表皮生长因子受体2 临床病理特征 

分 类 号：R373.9[医药卫生—病原生物学] R730.43[医药卫生—基础医学]