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作 者:郭金丽[1] 陈贵华[1] 樊丽[1] 朱冠宇[1] 刘欢[1] 李连国[1]
机构地区:[1]内蒙古农业大学农学院,内蒙古呼和浩特010019
出 处:《北方园艺》2016年第20期18-21,共4页Northern Horticulture
基 金:国家自然科学基金资助项目(31260455)
摘 要:以‘红颜’草莓果实为试材,研究了果实采后衰老过程中线粒体呼吸相关酶活性及能量水平的变化,以了解能量与草莓果实衰老的关系。结果表明:随着草莓果实的衰老,草莓果实线粒体琥珀酸脱氢酶(SDH)、H+-ATPase和Ca2+-ATPase活性均明显下降,细胞色素氧化酶(CCO)活性上升;三磷酸腺苷(ATP)含量持续下降,二磷酸腺苷(ADP)和磷酸腺苷(AMP)含量上升;ATP、ADP、AMP 3种能量物质含量差异明显,其中ATP含量远高于ADP和AMP含量,ATP含量代表了草莓果实中的能量水平,且能荷(EC)与ATP协同变化;试验表明,草莓果实衰老与线粒体呼吸相关酶活性下降、线粒体功能下降而导致的能量不足有关。The dynamic variation of mitochondria respiratory metabolism-related enzyme activities and energy level were studied during aging process in‘Hongyan'strawberry fruit in order to understand the relationship between energy and aging of strawberry fruit.The results showed that,in storage process of strawberry fruit,the activities of mitochondria respiratory metabolism-related enzyme succinic dehydrogenase(SDH),H+-ATPase,Ca2+-ATPase decreased obviously,cytochrome oxidase(CCO)activity increased.ATP content decreased continuously,contents of ADP,AMP increased,contents of three energy level were different and ATP content was much higher than contents of ADP and AMP,ATP content was the dominant energy resource in fruit.Energy charge had similar trend with ATP.In conclution,decline of activities of mitochondria respiratory metabolism-related enzyme stated the decline of mitochondria function which resulted in energy deficiency.Energy deficiency played a role in aging of strawberry fruit.
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