应力环境对PDU中膀胱起搏细胞的密度影响  

The effect of stress environment on the density of bladder pacemaker cells in PDU

作　　者：李云飞[1] 张少峰[1] 甘伟[1] 张正龙[1] 季辉华[1] 陈胜[1] 王天宝[1] 刘云[1] 

机构地区：[1]湖北医药学院附属人民医院泌尿外科,十堰市442000

出　　处：《实用医学杂志》2016年第19期3151-3154,共4页The Journal of Practical Medicine

基　　金：湖北省卫生计生委基金资助项目(编号:WJ2015MB220);十堰市科技局资助项目(编号:15Y32)

摘　　要：目的:探讨前列腺增生合并逼尿肌收缩无力(PDU)中膀胱起搏细胞(ICC)的密度及应力环境对其生长的影响。方法:采用丙酸睾丸酮联合尿动力学方法建立PDU豚鼠模型(n=23),以同质前列腺增生(BPH,n=6)及正常豚鼠(n=20)作为对照,然后将三组膀胱制作冰冻切片,应用免疫荧光染色及共聚焦显微镜观察ICC数目变化。进一步通过体外流体应力培养ICC,结合细胞克隆形成实验动态观察应力环境对ICC生长数目的影响。结果:PDU膀胱组织中ICC数目[(0.28±0.52)%]与正常对照组[(5±0.36)%],及BPH组[(5.4±0.42)%],比较明显减少,其中以二聚体ICC减少更为显著,它与单体ICC比例在PDU组(6.13±1.45)%明显低于正常对照组[31.55±9.67)%,P<0.05]和BPH组[71.52±8.95)%,P<0.05]。体外ICC生长密度与流体应力的作用时间呈负相关(r=-0.714 9),去应力环境后的培养持续时间与ICC密度呈正相关(r=0.815 7)。结论:PDU中存在ICC密度分布异常,ICC数目减少与应力环境的作用时间有关,提示尽早消除膀胱中的应力环境可能有助于恢复PDU中的ICC密度。Objective To investigate the density of bladder pacemaker cells （ICC） in prostatic hyperplasia with detrusor underactivity （PDU） and the impact of stress environment on its growth. Methods 23 PDU guinea pig models and 26 pigs for control （BPH, n = 6; normal group, n = 20） were developed. After water-filled urodynamic pressure measurement of bladder for three groups , detrusor tissue was made into frozen sections. TheICC numberwere measured with immunofluorescence stainingand observed by confocal microscopy. Further ICC cells in vitro were cultured under stress environment and its counts were analyzed by colony formation assay. Results Decreased density of ICC was observed in PDU group （0.28 ± 0.52）% compared to control [（5 ± 0.36）%,P 〈 0.05] and the BPH group （5.4% ± 0.42）%, P 〈 0.05）. The ratio of dimer and monomer [（6.13 ± 1.45）%] in PDU group was significantly lower than those of the control [31.55 ± 9.67）%, P 〈 0.05] and the BPH group [（71.52 ± 8.95）%, P 〈 0.05]. There is a negative correlation between the density of ICC in vitro and duration of stress. By contrast , there is a positive correlation between the density of ICC and incubation time after the stress is removed. Conclusion Distribution of decreased density of ICC is involved in the pathogenesis of PDU , which may be due to long-term stress environment. Thus eliminating stress environment may help restore ICC density in PDU.

关 键 词：CAJAL间质细胞 逼尿肌 良性前列腺增生 应力 

分 类 号：R697.3[医药卫生—泌尿科学]