混合三模板分子印迹固相萃取柱应用于粮谷中三唑类农药的检测  

Application of Mixing Three-Template Molecularly Imprinted Solid Phase Extraction Column to Determin Triazole Pesticides in Grains

作　　者：高林[1,2] 李亚丽[1,2] 刘博[1,3] 高文惠[1,2] 

机构地区：[1]河北科技大学生物科学与工程学院,石家庄050000 [2]河北省发酵工程技术研究中心,石家庄050000 [3]张家口卷烟厂有限责任公司,张家口075000

出　　处：《中国粮油学报》2016年第10期131-136,共6页Journal of the Chinese Cereals and Oils Association

基　　金：河北省科技支撑计划(14227504D);河北省食品药品监督管理局食品药品安全科技计划(ZD2015015)

摘　　要：建立了采用混合三模板分子印迹固相萃取-高效液相色谱法分离检测粮谷样品中3种三唑类杀菌剂残留的方法。以联苯三唑醇、腈菌唑、烯唑醇为混合模板分子，采用本体聚合法合成了具有高选择性的混合三模板分子印迹聚合物，以该聚合物为填料制备混合三模板分子印迹固相萃取柱，并结合高效液相色谱法检测粮谷中联苯三唑醇、腈菌唑、烯唑醇残留。结果表明，以20mL水为淋洗剂，15mL甲醇为洗脱剂，并在最佳色谱条件下，联苯三唑醇、腈菌唑、烯唑醇的平均回收率分别为74．0％～82．4％、79．0％～86．2％、77．5％～85．1％，相对标准偏差（RSD）≤4．4％，该混合三模板分子印迹聚合物对3种杀菌剂具有特异性吸附能力，该分析方法可用于样品中三唑类杀菌剂残留的分离检测。A mixing three -template molecularly imprinted solid phase extraction column （MISPEC） -high performance liquid chromatography （HPLC） method was developed for simultaneous determination of three triazole fungicide residues in grain samples. The mixing three -template molecularly imprinted polymer （MIP） with high selectivity was synthesized through bulk polymerization using bitertanol, myclobutanil and diniconazole as mixing three - template molecules. On this basis, the mixing three - template molecularly imprinted solid phase extraction column was prepared and used coupled with HPLC to detect bitertanol, myclobutanil and diniconazole in grains. The experi- mental results showed that with water of 20 mL as washing agent, methanol of 15 mL as eluent, and under the best chromatographic conditions, the average recoveries of bitertanol, myclobutanil and diniconazole were respectively 74.0%- 82.4%, 79.0%- 86.2% and 77.5%-85. 1% with the relative standard deviations （RSD） less than 4.4%. In conclusion, the polymer had a good specificity absorption capacity for the three fungicides, and the analytical method could be used for the separation and detection of triazole fungicides in samples.

关 键 词：粮谷 三唑类农药 混合模板 分子印迹固相萃取柱 高效液相色谱法 

分 类 号：O652.6[理学—分析化学]