电针穴位对分娩大鼠镇痛效应及5-HT mRNA与蛋白表达的影响  

Effects of Electric Acupuncture Acupoints for Childbirth Analgesia Rats and 5-HT mRNA and Protein Expression

作　　者：蒋秋燕[1] 王美丽[1] 李丽[1] 李艳林[1] 王梦莹[1] 

机构地区：[1]广西中医药大学第一附属医院,南宁530023

出　　处：《中国中医基础医学杂志》2016年第10期1376-1379,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：2012年国家自然科学基金资助项目(81260547)-不同穴位电针对分娩疼痛相关神经递质mRNA及蛋白表达影响的研究

摘　　要：目的:探讨电针穴位对大鼠分娩的镇痛效应及其中枢5-HT、2A受体基因与蛋白的表达。方法:120例孕鼠按随机数字表法分为空白组、电针三阴交穴组、电针合谷穴组、电针合谷加三阴交组、电针血海穴组及药物组。热水甩尾观察镇痛效应,ELISA法检测血清5-HT水平,Real-time PCR、Westernblot检测中枢5-HT及2A受体mRNA与蛋白表达。结果:大鼠痛阈值治疗前(F=0.736)组间比较差异无统计学意义;治疗后(F=216.361)组间比较差异有统计学意义;血清5-HT、大脑、脊髓5-HT及2A受体mRNA与蛋白表达组间比较差异有统计学意义。电针不同穴位由低至高依次为空白组<血海组<合谷加三阴交组<合谷组<三阴交组<药物组,不同程度降低血清5-HT表达水平,提高大鼠痛阈值及大脑、脊髓5-HT及2A受体mRNA与蛋白表达水平。结论:电针不同穴位产生不同针效作用与电针不同程度降低血清5-HT表达水平及提高中枢5-HT、2A受体mRNA与蛋白表达相关,揭示5-HT及2A受体参与电针穴位镇痛的作用机制。Objective： To explore the analgesic effect of electric acupuncture on labor of pregnant rat,mRNA and protein of central 5-HT and receptor,Method： 120 successful models of pregnant rats into control group,model group,Sanyinjiao group,Hegu group,Hegu plus Sanyinjiao group,Xuehai group,Meperidine group. observing the analgesic effect through hot flick method of measurement of pain. Detecting the serum 5-HT level by ELISA method,detect expression of the rat central 5-HT and receptor mRNA and Western blot by Real-time PCR. Results： Before treatment,the differences of pain threshold among the groups are not statistically significant（ F = 0. 736）; After treatment（ F = 216. 361）,there were statistically significant differences between groups. Serum 5-HT,cerebral spinal 5-HT mRNA and protein 2A receptor,and,significant differences between the groups was statistically significant. Electrical Acupuncture from low to high were model group Xuehai group Hegu plus ＋ Sanyinjiao group Hegu group Sanyinjiao group Meperidine group. Varying degrees of reduction of serum expression levels of 5-HT. Improving and increasing the pain threshold of rat brain,spinal cord and the 5-HT 2A receptor mRNA and protein expression levels. Conclusion： Electrical Acupuncture needles have different effects and EA effect to vary degrees to reduce serum levels of 5-HT expression and improve the central 5-HT,2A receptor mRNA and protein expression associated reveals 5-HT 2A receptor is involved and EA acupuncture analgesia mechanism.

关 键 词：电针穴位 分娩镇痛 5-HT及2A受体 mRNA与蛋白表达 

分 类 号：R245.97[医药卫生—针灸推拿学]