三种水生动物细胞系对两株蛙病毒敏感性的比较  

Comparative susceptibility of three aquatic animal cell lines to two ranaviruses

作　　者：雷存科 陈中元[1,2] 张奇亚[1,2] 

机构地区：[1]中国科学院水生生物研究所,淡水生态与生物技术国家重点实验室,湖北武汉430072 [2]中国科学院大学,北京100049

出　　处：《水产学报》2016年第10期1643-1647,共5页Journal of Fisheries of China

基　　金：国家自然科学基金(31430091)

摘　　要：利用3个不同物种的水生动物细胞系,包括爪蟾肾细胞系(A6)、大鲵胸腺细胞系(GSTC)和鲤上皮瘤细胞系(EPC),分别用沼泽绿牛蛙蛙病毒(RGV)和大鲵蛙病毒(ADRV)感染,进一步研究细胞病变显微形态、病毒滴度、细胞病变与不同感染时间的相关性等。结果显示,在光镜下可见感染病毒的细胞发生病变,A6和EPC细胞肿胀或破裂;GSTC细胞收缩或聚在一起形成多层。同种水产动物细胞系对不同蛙病毒的敏感性不同,在A6、EPC和GSTC细胞中,RGV的滴度分别为10^(3.6)、10^(5.9)和10^(6.6) TCID_(50)/m L;ADRV的滴度分别为10^(4.3)、10^(5.4)和10^(6.1) TCID_(50)/m L,表明GSTC细胞系对两种蛙病毒都更敏感。研究为后续蛙病毒致病机理提供了有用的信息和重要实验材料。Ranaviruses not only infect frogs but can also infect many other aquaculture animals, such as fish,amphibians and reptiles. Here, three aquatic animal cell lines, including Chinese giant salamander thymus cell line（GSTC）, Epithelioma papulosum cyprini cell line（EPC） and Xenopus kidney cell line（A6）, were infected with two raninvirus strains, Rana grylio virus（RGV） and Andriasda davidianus ranavirus（ADRV）, respectively. After the ranavirus infection, the morphological changes in these three cell lines were observed by light microscope. The pathological changes and virus titer were recorded and compared at different time post infection. Microscopic observation showed that A6 cells and EPC cells swelled and ruptured. GSTC cells shrank and gathered together to multilayer. The virus titers of two ranaviruses varied significantly in different cells lines. For RGV, 103.6, 105.9 and106.6TCID50/m L in A6, EPC and GSTC cells respectively. For ADRV, 104.3, 105.4 and 106.1 TCID50/m L in A6, EPC and GSTC cells, respectively. These results showed that the susceptibilities of the same aquatic animal cell lines to different ranavirues were different. This suggested that GSTC is the most sensitive cell line to the ranaviruses. This study provides valuable information and materials for the further study of the pathogenesis of ranaviruses.

关 键 词：沼泽绿牛蛙蛙病毒 大鲵蛙病毒 爪蟾肾细胞系 大鲵胸腺细胞系 鲤上皮瘤细胞系 细胞病变 

分 类 号：S941.41[农业科学—水产养殖]