丙泊酚对兔脊髓缺血再灌注损伤血脊髓屏障的保护作用  

The protection by Propofol for injured blood-spinal cord barrier in spinal cord ischemia reperfusion in rabbits

作　　者：解立杰 黄锦秀[1] 胡霁[1] 

机构地区：[1]华中科技大学同济医学院附属梨园医院麻醉科,武汉市430077

出　　处：《实用医学杂志》2016年第20期3364-3368,共5页The Journal of Practical Medicine

基　　金：湖北省自然科学基金项目(编号:2013CFB086);中央高校基本科研业务费专项资金资助项目(编号:2016YXZDO24)

摘　　要：目的:探讨丙泊酚对兔脊髓缺血再灌注损伤血脊髓屏障的保护作用。方法:72只日本大耳白兔随机分为3组:假手术组(Sham)、模型组(I/R)、丙泊酚组(I/R+P)。I/R和I/R+P组阻断腹主动脉30 min。I/R+P组于主动脉阻断前10 min和再灌注即刻泵注丙泊酚,另两组泵注生理盐水。再灌注24、48 h行神经功能评分,EB检测血脊髓屏障的通透性,Western blot,RT-PCR检测MMP-9、claudin-5、NF-κB表达,HE染色观察形态变化。结果:与Sham组相比,I/R组神经功能评分降低,屏障通透性增加,MMP-9、NF-κB表达增多,claudin-5表达减少(P<0.05);与I/R组相比,I/R+P组神经功能评分增高,屏障通透性降低,MMP-9、NF-κB表达减少,claudin-5表达增多(P<0.05)。结论:丙泊酚对兔脊髓缺血再灌注损伤血脊髓屏障具有保护作用,其保护机制可能与抑制NF-κB信号通路,抑制MMP-9的表达,减少claudin-5的丢失有关。Objective To investigate the effects of Propofol in blood spinal cord barrier（BSCB） disruption induced by spinal cord ischemia reperfusion injury（SCIRI）. Methods 72 Japanese white rabbits were randomly assigned into 3 groups： sham-operation group（S）; ischemia / reperfusion group（I / R） and Propofol treatment group（I / R ＋ P）. The Group S was separated the aorta without cross-clamping. SCIRI was induced in rabbits by infrarenal aortic occlusion for 30 minutes. Propofol was intravenously infused at 10 minutes before aortic clamping and at onset of reperfusion in the Group I / R ＋ P. The Group S and Group I / R were intravenously infused 0.9% sodium chloride. Hind-limb motor function was assessed using Tarlov criteria, and histological observation by histological examination. The permeability of the BSCB was examined using EB as vascular tracers. The expression of MMP-9, claudin-5 and NF-κB were assessed by Western blot, RT-PCR. Results Propofol minimized the neuromotor dysfunction and histopathological deficits and attenuated EB extravasation. In addition, Propofol suppressed SCIRI-induced increase of MMP-9 and NF-κB. Finally, Propofol reduced the loss of claudin-5.Conclusion Propofol stabilizes the BSCB integrity after SCIRI. This beneficial effect is partly mediated by inhibition of MMP-9 and preservation claudin-5 and relates to inhibiting the NF-κB signal pathway.

关 键 词：再灌注损伤 脊髓缺血 丙泊酚 血脊髓屏障 

分 类 号：R614[医药卫生—麻醉学]