遵义汉族载脂蛋白A5(c.553G/T)基因多态性与高甘油三酯血症  

Association of Apolipoprotein A5(c.553G/T) gene polymorphism with hypertriglyceridemia of the Han Nationality in Zunyi

作　　者：余晓[1] 戴华玉[2] 王业忠[2] 耿争光 张志敏[3] 

机构地区：[1]遵义医学院生物化学教研室,563099 [2]遵义医学院,2012级临床医学专业5603099 [3]遵义医学院遗传学教研室,563099

出　　处：《实用医学杂志》2016年第20期3386-3390,共5页The Journal of Practical Medicine

基　　金：贵州省科技厅项目(编号:黔科合J字LKZ[2012]39号);遵义医学院大学生创新创业训练计划项目(编号:院发[2014]5828)

摘　　要：目的：探讨ApoA5基因c.553G/T多态性与遵义汉族HTG相关性。方法：用PCR-RFLP技术检测分析103例HTG患者和165例正常对照者ApoA5 c.553G/T多态性及地区分布差异。结果：两组中ApoA5c.553G/T基因型频率有显著差异（P〈0.05）,c.553T基因在HTG组中频率明显高于正常组（P〈0.05）,对HTG具有独立影响（OR=15.768,95%CI：5.916～42.025,P〈0.001）;与我国汉族正常人群比较明显低于台湾、江苏和湖北（P〈0.05）,与湖南和新疆相似（P〉0.05）;在HTG人群中,与江苏相似（P〉0.05）,明显高于新疆、低于台湾（P〈0.05）。结论：ApoA5 c.553G/T多态与遵义汉族HTG发病存在相关性及地区差异,c.553T基因可能是HTG的独立危险因素。Objective To investigate the relativity between c.553 G / T polymorphism in exon 4 of Apolipoprotein A5 gene and hypertriglyceridemia（HTG） in Zunyi Han Nationality. Methods c.553 G / T polymorphism of 103 HTG patients and 165 healthy individuals were tested by polymerase chain reaction and restriction fragment length（PCR-RFLP） assay. The distributions of genotypes and allele frequencies in HTG patients and healthy group were analyzed between Zunyi population and others. Results The genotype frequency of the ApoA5 gene c.553 G / T showed statistical difference between patients group and normal groups（P〈0.05）.The distribution of ApoA5 c.553 T gene in HTG was higher than the normal group significantly（P〈0.05）, and it had effect on triglyceridemia level independently（OR = 15.768, 95% CI： 5.916 ～ 42.025, P〈0.001）. In the normal Han nationality groups, gene frequency of Zunyi was lower than that in Taiwan, Jiangsu and Hubei（P〈0.05）, but similar to that in Hunan and Xinjiang（P〉0.05）. In HTG groups, gene frequency of Zunyi was similar to that in Jiangsu（P〉0.05）, but higher than that in Xinjiang（P〈0.05） and lower than that in Taiwan（P〉0.05）. Conclusion There is relativity between ApoA5 gene c.553 G / T polymorphisms and HTG in Zunyi Han nationality and the differences vary across different areas. It could be an independent risk factor for HTG.

关 键 词：高甘油三酯血症 载脂蛋白A5基因 c.553G/T多态 遵义汉族人群 

分 类 号：R589.2[医药卫生—内分泌]