白及多糖对血热出血模型大鼠的止血作用及机制研究  

Study on Hemostatic Effect and Mechanism of Polysaccharides from Bletilla striata in Blood Heat and Hemorrhage Model Rats

作　　者：董永喜[1,2] 刘星星[3] 董莉[1,2] 王霞[1,2] 黄勇[2] 王永林[2] 

机构地区：[1]贵州医科大学药学院,贵阳550025 [2]贵州医科大学贵州省药物制剂重点实验室,贵阳550004 [3]贵阳市妇幼保健院,贵阳550003

出　　处：《中国药房》2016年第31期4347-4350,共4页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81360636;81460630);贵州省优秀青年科技人才专项(No.黔科合人字[2015]11号);贵州省高等学校创新能力提升计划(No.黔教合协同创新字[2013]04)

摘　　要：目的:研究白及多糖(BSP)对血热出血模型大鼠的止血作用及机制。方法:将大鼠随机分为正常对照组、模型组、云南白药组(0.50 g/kg)和BSP低、中、高剂量组(0.34、0.68、1.35 g/kg),每组10只。除正常对照组外,其余各组大鼠均采用干姜水煎液结合5%乙醇给药复制血热出血模型。造模成功后,除正常对照组和模型组大鼠ig生理盐水外,其余各组大鼠ig相应药物,每天1次,连续14 d。末次给药1 h后检测各组大鼠体质量、摄食量、饮水量、血小板聚集率、组织型纤溶酶原激活剂(t-PA)和纤溶酶原激活物抑制剂(PAI-1)的含量;检查肺、胃组织病理学变化。结果:与正常对照组比较,模型组大鼠体质量、摄食量、血小板最大聚集率、PAI-1含量均减少,饮水量、t-PA含量及t-PA/PAI-1比值均增加(P<0.05或P<0.01),肺、胃组织均表现出出血和炎症等病理变化。与模型组比较,云南白药组和BSP低、中、高剂量组大鼠体质量、摄食量均增加,饮水量减少(P<0.05或P<0.01);云南白药组和BSP中、高剂量组大鼠血小板最大聚集率、PAI-1含量均增加,t-PA/PAI-1比值减少(P<0.05或P<0.01),肺、胃组织的病理变化均不同程度减轻。结论:BSP可能通过升高血小板聚集率、调节纤溶系统、改善肺和胃组织的病变,综合发挥止血作用。OBJECTIVE： To study the hemostatic effect and mechanism of the polysaccharides from Bletilla striata in blood heat and hemorrhage （BBH） model rats. METHODS： Rats were randomly divided into normal control group, model group, Yunnan baiyao group （0.50 g/kg）, and BSP low-dose, medium-dose and high-dose groups （0.34, 0.68, 1.35 g/kg）, with 10 rats in each group. Except for normal control group, those groups were given water decoction of Rhizoma Zingiberis and 5% ethanol to induce BBH model. After modeling, those groups were given relevant medicine intragastrically, once a day, for 14 days, except normal control group and model group were given normal saline intragastrically. 1 h after last administration, body weight, food intake, water intake, the rate of platelet aggregation, the contents of t-PA and PAI-1, pathological change of lung and stomach were all detected in each group. RESULTS： Compared with normal control group, body weight, food intake, maximal rate of platelet aggregation and the content of PAI-1 were decreased in model group; while water intake, the content of t-PA and ratio of t-PA/PAI-1 were increased （P〈0.05 or P〈0.01）. Moreover, hemorrhage and inflammation of lung and stomach were found. Compared with model group, the body weight and food intake of Yunnan baiyao group, BSP low-dose, medium-dose and high-dose groups were increased, while water intake were decreased （P〈0.05 or P〈0.01） ; the maximal rate of platelet aggregation and the content of PM-1 were increased in Yunnan baiyao group, BSP medium-dose and high-dose groups, while ratio of t-PA/PAI-1 were decreased （P〈0.05 or P〈0.01）, the pathological changes of lung and stomach relieved to varying degrees. CONCLUSIONS： BSP exerts hemostatic effect by up-regulating the rate of platelet aggregation, rising fibrinolysis systems, improving the lesion of lung and stomach.

关 键 词：白及多糖 血热出血 大鼠 血小板聚集 纤溶系统 组织病理学检查 

分 类 号：R985[医药卫生—药品]