人参皂甙Rbl对大鼠局灶性脑缺血细胞凋亡的抑制作用  

Inhibition of Ginsenosides Rbl on Focal Cerebral Ischemia and Apoptosis

作　　者：袁秀梅[1] 李思瓯[2] 尹昌浩[1] YUAN Xiumei Li Siou YIN Changhao(Department of Neurology, Hongqi Hospital of Mudanjiang Medical University, Helongjiang Province, Mudanjiang 157003, China Department of Endocrinology, Iqongqi Hospital of Mudanjiang Medical University, Helongjiang Province, Mudanjiang 157003, China)

机构地区：[1]牡丹江医学院红旗医院神经科,牡丹江157003 [2]牡丹江医学院红旗医院内分泌科,牡丹江157003

出　　处：《中国中医药现代远程教育》2016年第20期138-140,共3页Chinese Medicine Modern Distance Education of China

基　　金：牡丹江医学院科研项目资助(No:2010-06)

摘　　要：目的探究人参皂甙Rbl对大鼠局灶性脑缺血细胞凋亡的抑制作用。方法首先建立一个阻塞大鼠大脑局灶性暂时脑缺血的模型,将神经功能出现缺失症的大鼠按照随机的原则分别分为GRb1组与缺血组,然后对灌注后的GRb1组大鼠进行人参皂甙Rbl腹腔注射,以45 mg/kg为宜,对这些大鼠进行不同时间阶段的再灌注,按照时间点的不同,将GRb1组大鼠分为7个组别,然后用免疫组织化学法以及原位末端标记法对大鼠的细胞凋亡情况进行观察分析。结果经过参皂甙Rbl干预的GRb1组大鼠与缺血组相比,其各分组内的大鼠凋亡细胞数量降低,且仅在灌注的12 h^3 d时间阶段内的降低数量差异比较明显,NAIP阳性细胞数在进行再灌注12 h^10 d与缺血组有着明显的差别,Bcl-2阳性细胞数在灌注12 h^10 d期间出现了明显的上升趋势,且与缺血组大鼠相比,Bax阳性细胞数在相同的时间点出现下降。结论采用人参皂甙Rbl能够通过对NAIP与Bcl-2的促进起到对大鼠的局灶性脑缺血细胞凋亡的保护作用,可以在临床医学中得以广泛地推广、应用。Objective To explore the inhibition of ginsenosides Rbl on focal cerebral ischemia and apoptosis. Methods Firstly, a cerebral occlusion model of focal cerebral ischemia in temporarily was established. The neurological deficit disorder occurs in rats in accordance with the principle of random were divided into GRb1 group and ischemia group. After perfusion, the GRb1 group with large ginsenosides Rbl mice was injected intraperitoneally with 45 mg / kg. These rats were given reperfusion in different period of time, according to different points in time, and the rats were divided into seven groups GRb1 groups. The immunohistochemistry TUNEL method was used to observe and analyze cell apoptosis of rats. Results After the intervention of ginsenoside Rbl GRb1 rats with ischemia group compared to the number of apoptotic cells in rats which each packet was lowered, and only reduce the number of differences in perfusion 12 h ~ 3d period of time within the more obvious, NAIP positive cells during reperfusion 12 h ~ 10 d and is-chemic group has a significant difference, Bcl-2 positive cells in perfusion has a clear upward trend during the 12 h ~ 10 d, and compared with ischemic rats, Bax positive cells decline at the same point in time. Conclusion Ginsenoside Rbl through NAIP and promoting Bcl-2 plays a protective effect on focal cerebral ischemia in rat＇s apoptosis, and can be widely promotion and application in clinical medicine.

关 键 词：人参皂甙RB1 大鼠 局灶性脑缺血 细胞凋亡 抑制 动物实验 中风 

分 类 号：R285.5[医药卫生—中药学]