高效液相色谱法对降脂通便胶囊中大黄有效成分测定  

Determination on Effective Components of Rhubarb in Jiangzhi Tongbian Capsule by High Performance Liquid Chromatography

作　　者：孟晓丹 MENG Xiaodan(Department of Pharmacy, Luoyang Third People＇s Hospital, Henan Province, Luoyang 471000, China)

机构地区：[1]河南省洛阳市第三人民医院药剂科,洛阳471000

出　　处：《中国中医药现代远程教育》2016年第20期140-141,共2页Chinese Medicine Modern Distance Education of China

摘　　要：目的对降脂通便胶囊中大黄有效成分进行含量测定。方法应用高效液相色谱法测定本院制剂降脂通便胶囊有效成分大黄酚及大黄素的含量。结果大黄素的回归方程为A1=3685.0C1-2.6838(r=0.9998),线性范围为0.0129μg^0.226μg;大黄酚的回归方程为A2=5695.6C2-5.2986(r=0.9998),线性范围为0.0173μg^0.302μg。结论高效液相色谱(HPLC)法用于我院制剂降脂通便胶囊的含量测定控制。该方法简便易行,重现性好。可用于测定降脂通便胶囊中的大黄素和大黄酚的含量。Objective To determine the content of effective components of Rhubarb in Jiangzhi Tongbian capsule. Methods The high performance liquid chromatography（HPLC） was used to determine the effective components of rhubarb and emodin in our hospital.Results The regression equation of emodin was A1=3685.0C1-2.6838（r=0.9998）, the linear range was 0.0129 mu g to 0.226 mu g,the regression equation was A2=5695.6C2-5.2986（r=0.9998）, and the linear range was 0.0173 mu g to 0.302 Mu G. Conclusion The HPLC was used for the determination of the content of the capsule in the preparation of our hospital. This method is simple and easy to operate, and has good reproducibility. It can be used to determine the content of emodin and rhubarb in Jiangzhi Tongbian capsule.

关 键 词：降脂通便胶囊 高效液相色谱 大黄素 大黄酚 含量测定 

分 类 号：R286.0[医药卫生—中药学]