炮制对补骨脂中12种化学成分含量的影响  

Effect of Processing on Contents of Twelve Constituents in Psoraleae Fructus

作　　者：陶益[1] 蒋妍慧 李伟东[1] 蔡宝昌[1] 

机构地区：[1]南京中医药大学江苏省中药炮制重点实验室,南京210023

出　　处：《中国实验方剂学杂志》2016年第21期6-9,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2012ZX09304005);江苏省自然科学基金青年项目(BK20140963)

摘　　要：目的:比较补骨脂生品及3种炮制品的各项检查指标及化学成分的差异,为补骨脂不同炮制品的临床使用提供参考。方法:按《中国药典》2015年版标准,测定补骨脂生品、炒品、盐炙品及酒炙品的杂质、水分、总灰分和酸不溶性灰分,通过HPLC比较补骨脂不同炮制品中主要化学成分的差异。结果:补骨脂生品及3种炮制品的杂质、水分、总灰分、酸不溶性灰分测定结果均符合《中国药典》2015年版的要求。通过对照品比对及文献查阅确定了12个主要化学成分,即补骨脂苷,异补骨脂苷,补骨脂素,异补骨脂素,新补骨脂异黄酮,补骨脂甲素,补骨脂定,补骨脂乙素,补骨脂二氢黄酮甲醚,corylifol A,4'-O-甲基补骨脂查尔酮和补骨脂酚。结论:补骨脂酚有肾毒性和肝毒性,炮制后补骨脂酚在酒炙品和盐炙品中含量均较生品下降,说明酒炙和盐炙具有炮制减毒的作用;与生品相比,盐炙品中补骨脂素和异补骨脂素含量显著升高,这与补骨脂盐炙后温肾助阳作用增强相关。4'-O-甲基补骨脂查尔酮在炮制品中含量显著升高,而补骨脂二氢黄酮甲醚含量显著降低,这可能是由补骨脂二氢黄酮甲醚在高温下发生降解转化而成。上述这些成分的改变可能是不同炮制品临床功效产生差异的主要原因。Objective： To compare quality control indexes and chemical compositions of crude Psoraleae Fructus and its three processed products. Method： Impurity,water content,total ash and acid insoluble ash of different processed products were performed in accordance with standards in the 2015 edition of Chinese Pharmacopoeia. HPLC was established to compare contents of major ingredients in crude Psoraleae Fructus and its different processed products. Result： The impurity, water content, total ash and acid insoluble ash of crude Psoraleae Fructus and its three processed products met requirements of the 2015 edition of Chinese Pharmacopoeia.Twelve major constituents were identified. Conclusion： Bakuchiol has renal toxicity and liver toxicity. After processing,the content of bakuchiol decreases, indicating that wine and salt processed products can attenuate toxicity of crude products; psoralen and isopsoralen significantly increase, these two components can promote growth of osteoblasts,which was in consistent with theory that salt processing can enhance kidney-Yang effect.The content of 4＇-O-methylbroussochalcone B significantly increases in processed products,whereas bavachinin A significantly reduces,which may be due to that bavachinin A degrades and turns into 4＇-O-methylbroussochalcone B at high temperatures. Variations in contents of these constituents in Psoraleae Fructus may contribute to different clinical efficacy of its processed products.

关 键 词：补骨脂 炒品 酒炙品 盐炙品 补骨脂素 异补骨脂素 

分 类 号：R283.1[医药卫生—中药学] R943.1[医药卫生—中医学]